家畜生态学报2024，Vol.45Issue(8)：33-40,8.DOI:10.3969/j.issn.1673-1182.2024.08.006
仔猪初生重对肌肉血管生成及代谢组学的影响
Effects of Birth Weight on Muscle Angiogenesis and Metabolic Profiling in Piglets
摘要
Abstract
This study was conducted to investigate the effects of birth weight on muscle angiogenesis in piglets.Twenty newborn Tunchang black piglets were divided into two groups based on their body weight:normal birth weight piglets(NBW,1162±98 g)and low birth weight piglets(LBW,724±31 g),with 10 piglets in each group.The semitendinosus muscles of the piglets were collected for angiogenesis and me-tabolite determination.The results showed as follows:1)compared with NBW group,LBW group exhibi-ted significantly decreased protein expression levels of the angiogenesis markers platelet-endothelial cell ad-hesion molecule 1(CD31),vascular endothelial growth factor A(VEGF-A),and matrix metalloproteinase 9(MMP9)in semitendinosus muscle(P＜0.05).2)To further investigate the metabolites related to mus-cle angiogenesis,the metabolomics technology was employed to screen differential metabolites in the mus-cle of the two groups.It was found that differential metabolites in the semitendinosus muscle of NBW and LBW piglets were mainly enriched in glycerophospholipid metabolism,unsaturated fatty acid metabolism pathways,arginine and proline metabolism pathways.In the glycerophospholipid metabolism pathway,metabolites such as choline,phosphatidylserine,2-acyl-sn-glycerol-3-phosphor-serine,and 1-acyl-sn-glyc-erol-3-phosphate choline were significantly increased in the LBW group(P＜0.05);in the unsaturated fat-ty acid metabolism pathway,the level of linoleic acid was significantly decreased and that of stearic acid were significantly increased in the LBW group(P＜0.05);in the arginine and proline metabolism path-way,the levels of S-adenosyl-L-methionine and ornithine in muscle were significantly decreased in the LBW group(P＜0.05).These results suggest that the decreased muscle angiogenesis in LBW piglets may be associated with changes in metabolites in the glycerophospholipid metabolism pathway,arginine and proline metabolism pathway,and unsaturated fatty acid metabolism pathway.关键词
血管生成/半健肌/代谢组学/仔猪/低初生重Key words
angiogenesis/semimembranosus/metabolomics/piglets/low birth weight分类
农业科技引用本文复制引用
邹冬彬,杨运,吕仁龙,周汉林,胡诚军..仔猪初生重对肌肉血管生成及代谢组学的影响[J].家畜生态学报,2024,45(8):33-40,8.基金项目
海南省自然科学基金青年基金项目(321QN320) （321QN320）
中央级公益性科研院所基本科研业务费专项(1630032023009) （1630032023009）
海南省地方猪现代农业产业技术体系(HNARS-2022-2-G03) （HNARS-2022-2-G03）
