家畜生态学报2024，Vol.45Issue(8)：41-46,6.DOI:10.3969/j.issn.1673-1182.2024.08.007
水苏糖对秦川牛精液冷冻保存效果的影响
Effect of Stachyose on the Cryopreservation of Qinchuan Bull Semen
摘要
Abstract
The experiment was to explore the effect of stachyose on semen cryopreservation of Qin-chuan bull.Different concentrations of stachyose(0 mg/mL,20 mg/mL,40 mg/mL,60 mg/mL and 80 mg/ml)were supplemented to the frozen diluent of bull semen,then the sperm motility,membrane integ-rity,acrosome integrity,mitochondrial activity and antioxidant properties related indicators of frozen-thawed semen in Qinchuan bull were tested.The results showed that 40 mg/mL stachyose treatment sig-nificantly(P＜0.05)improved sperm motility(43.83％)and acrosome integrity(78.70％)after freezing-thawing,compared with the control group;membrane integrity and mitochondrial activity were enhanced significantly with the increase of stachyose concentration(P＜0.05),but the membrane integrity was not significantly different between the different concentrations of stachyose(P＞0.05);in addition,the activi-ties of GSH-Px enzyme and CAT enzyme were significantly increased(P＜0.05),and the content of MDA significantly reduced(P＜0.05)in the 40 mg/mL stachyose.In summary,the 40 mg/mL stachyose treat-ment significantly improves the quality and antioxidant capacity of freezing-thawing semen,and has a good preservation effect on the frozen semen in Qinchuan bull.关键词
水苏糖/精液稀释液/冷冻保存/秦川牛/精液Key words
Stachyose/semen diluent/cryopreservation/Qinchuan bull/semen分类
农业科技引用本文复制引用
王萌,杜鑫泽,杨超群,贾鸿儒,昝林森,李付强,张涛平,杨武才..水苏糖对秦川牛精液冷冻保存效果的影响[J].家畜生态学报,2024,45(8):41-46,6.基金项目
陕西省杨凌区2021年试验示范站(基地)科技创新与成果转化项目(TGZX2021-25) （基地）
国家肉牛牦牛产业技术体系建设(CARS-37) （CARS-37）
陕西省畜禽育种"两链融合"重点专项(2022GD-TSLD-46-0104) （2022GD-TSLD-46-0104）
