基础医学与临床2024，Vol.44Issue(8)：1094-1100,7.DOI:10.16352/j.issn.1001-6325.2024.08.1094
三七皂苷Rg1对阿尔茨海默症大鼠的神经系统的保护作用
Nervous protection provided by notoginsenoside Rg1 in rat model with Alzheimer's disease
摘要
Abstract
Objective To explore the mechanism of notoginsenoside Rg1 in preventing and treating Alzheimer's disease(AD).Methods Rat model of AD was established by injecting amyloid beta peptide 1-42(Aβ1-42)into the lateral ventricle of SD rats.Then the animals were randomly divided into three groups:sham-operated group,model group and Rg1 treatment group.The treatment group was treated with Rg1 gavage and the sham-operated group was treated with saline gavage.Learning and memory capacity of rats were examined by Morris water maze experiment(MWM).Moreover,the contents of MDA and SOD in cerebral cortex were detected by chemical colorimetry;immuno-histochemistry was used to detect caspase-3 protein in the cerebral cortex and Western blot was employed to detect the expression of p38 and p-p38 proteins.Results Compared with the sham-operated group,the model group had a prolonged escape latency,reduced stay time in the target quad-rant and reduced frequency of leaping over platform;increased MDA and decreased SOD in cerebral cortex;increased caspase-3 protein-positive neurons.The difference of p38 expression was not statistically significant and the phosphorylation of p38 was upregulated(P＜0.05).The rats in Rg1 treatment group had a shorter es-cape latency,increased stay time in quadrant Ⅲ,increased frequency of leaping over platform,decreased caspase-3 positive neurons and the phosphorylation level of p38,decreased MDA and increased SOD compared with the model group(P＜0.05).Conclusions Rg1 significantly improves learning and memory capacities,increases antioxidant capacity and plays neuro-protective effect in AD rat model by inhibition of p38 activation.关键词
三七皂苷Rg1/p38/细胞凋亡/神经系统Key words
notoginsenoside Rg1/p38/cell apoptosis/nervous system分类
医药卫生引用本文复制引用
王婷婷,魏欢,杨永丽,周贤,胡阳..三七皂苷Rg1对阿尔茨海默症大鼠的神经系统的保护作用[J].基础医学与临床,2024,44(8):1094-1100,7.基金项目
云南省科技厅昆明医科大学应用基础研究联合专项面上项目(202001AY070001-260,202201AY070001-188) （202001AY070001-260,202201AY070001-188）
昆明市卫生科技人才培养项目暨"千"工程后备入选(2019-03-07-003) （2019-03-07-003）
