基础医学与临床2024，Vol.44Issue(8)：1113-1119,7.DOI:10.16352/j.issn.1001-6325.2024.08.1113
敲降lncRNA UCA1降低人膀胱癌细胞系T24对吉西他滨耐药
Knockdown of lncRNA UCA1 reduces gemcitabine resistance of human bladder cancer cell line T24
摘要
Abstract
Objective To investigate the in vitro effect of lncRNA UCA1 on gemcitabine(GEM)resistance of blad-der cancer cell line T24 and its related molecular mechanism.Methods The mRNA expression of UCA1 in T24 cells and in T24/GEM cells was detected by RT-qPCR.The T24/GEM cells were incubated with varying concentra-tions(0.1,1,and 10 μmol/L)of GEM for 48 hrs.LC3 staining microscopy was employed to visualize autophagic puncta,while the expression of autophagy-related proteins was assessed by Western blot.UCA1-shRNA and UCA1-shRNA+pcDNA-Bcl-2 were transferred into T24/GEM cells,the sensitivity of cells to GEM was evaluated by MTT method and flow cytometry;the expressions of p53 and Bcl-2 were detected by Western blot.Results The expres-sion level of UCA1 in T24/GEM cells was significantly higher than that of parental T24 cells(P＜0.05).The con-centration of GEM in the range of 0-10 p.mol/L significantly induced dose-dependent autophagy in T24/GEM cells(P＜0.05).Knockdown of UCA1 enhanced the sensitivity of T24/GEM cells to GEM(P＜0.05),while reducing autophagy(P＜0.05)and down-regulating the expression of p53 and Bcl-2(P＜0.05).Over-expression of Bcl-2 partially reversed the GEM sensitization and autophagy inhibition of UCA1-shRNA in T24/GEM cells(P＜0.05).Conclusions Knockdown of lncRNA UCA1 reduces GEM resistance of T24/GEM cells by inhibiting Bcl-2 mediated autophagy.关键词
膀胱癌/lncRNA UCA1/Bcl-2/自噬/吉西他滨Key words
bladder cancer/lncRNA UCA1/Bcl-2/autophagy/gemcitabine分类
医药卫生引用本文复制引用
周昌东,林洋,孙凯,田玉新..敲降lncRNA UCA1降低人膀胱癌细胞系T24对吉西他滨耐药[J].基础医学与临床,2024,44(8):1113-1119,7.基金项目
吉林省卫健委基金支持项目(2021LC126) （2021LC126）
