基础医学与临床2024，Vol.44Issue(8)：1143-1148,6.DOI:10.16352/j.issn.1001-6325.2024.08.1143
白术内酯Ⅲ减轻脑梗死模型大鼠神经功能损伤
Atractylodes macrocephala lactone Ⅲ alleviates nerve function injury in rat model with cerebral infarction
摘要
Abstract
Objective To explore the effect of atractylodes macrocephala lactone Ⅲ(AML Ⅲ)on nerve injury of rats with cerebral infarction.Methods The rats were randomly divided into sham operation group,model group(modified Longa thrombus method to prepare rat cerebral infarction model),low and high dose of AML Ⅲ group(AML Ⅲ-L,AML Ⅲ-H)and high dose AML Ⅲ+EX527(SIRT1 inhibitor)group(AMLⅢ-H-EX527).Neuro-logical function was assessed by Zea-Longa score.Serum tumor necrosis factor(TNF-α)and interleukin(IL-6,IL-1β)were measured by ELISA;The level of super oxide dismutase activity(SOD),malonaldehyde content(MDA),and catalase activity(CAT)in brain tissue was detected by commercially available kit;pathological changes of hippocampus tissue and the area of cerebral infarction were observed by HE staining and TTC staining microscopy respectively.Apoptosis of brain cells was identified by TUNEL staining;protein expression of Bax,Bcl-2,SIRT1,and Nrf2 in rat brain tissue was detected by Western blot assay.Results After the intervention by AML Ⅲ,the pathological injury of hippocampus neurons in rats was alleviated.The neurological function score,serum TNF-α,IL-6 and IL-1 β levels,apoptosis rate,cerebral infarction size,MDA level and Bax protein expression were all decreased.SOD and CAT activity,SIRT1,Nrf2,Bcl-2 and protein expression were all in-creased(P＜0.05);EX527 was able to alleviate the protective effect of AML Ⅲ on neural function damage in rats with cerebral infarction.Conclusions Atractylodes macrocephala lactone Ⅲ reduces the nerve function injury in rat models with cerebral infarction.关键词
白术内酯Ⅲ/脑梗死/神经功能损伤/Zea-Longa评分Key words
macrocephala lactone Ⅲ/cerebral infarction/neurological function damage/Zea-Longa score分类
临床医学引用本文复制引用
张秋萍,杨月君,张彩丽..白术内酯Ⅲ减轻脑梗死模型大鼠神经功能损伤[J].基础医学与临床,2024,44(8):1143-1148,6.基金项目
保定市科技计划项目(17ZF152) （17ZF152）
