经济动物学报2024，Vol.28Issue(2)：113-120,8.DOI:10.13326/j.jea.2024.1745
维氏气单胞菌外膜蛋白OmpH功能预测及免疫原性分析
Function Prediction and Immunogenicity Analysis of Outer Membrance Protein OmpH of Aeromonas versonii
摘要
Abstract
The biological characteristics of the protein(OmpH)encoded by OmpH gene of Aeromonas veronii were analyzed,the dominant epitopes of B and T cells were predicted,the gene was cloned and prokaryotic expressed,and the immunogenicity of the expression product was analyzed in the study.The results showed that ompH gene of Aeromonas veronii was 513 bp in length,and the encoded protein had a transmembrane region,which was a hydrophilic outer membrane protein,and its secondary structure was mainly α-helix.Antigenic epitope prediction results showed that the B cell potential dominant antigenic peptide fragment of OmpH protein was 112-114 amino acids,and 7 Th and 6 CTL cell epitopes were predicted.Escherichia coli pColdTF-ompH prokaryotic expression strain was constructed,and OmpH protein was successfully expressed.The optimal induction time was 24 h,and the final concentration of the best inducer was 1.0 mmol/L.The results of Western-blot analysis showed that OmpH protein expressed after induction by recombinant bacteria could be recognized by rabbit anti-OmpH protein serum,which indicated that OmpH protein had good immunogenicity.关键词
维氏气单胞菌/外膜蛋白/OmpH基因/原核表达/免疫原性Key words
Aeromonas veronii/outer membrane protein/OmpH gene/prokaryotic expression/im-munogenicity分类
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李洪锦,赵桐,曹立男,康元环..维氏气单胞菌外膜蛋白OmpH功能预测及免疫原性分析[J].经济动物学报,2024,28(2):113-120,8.基金项目
吉林省自然科学基金项目(20210101050JC) （20210101050JC）
