中国当代医药2024，Vol.31Issue(18)：4-8,5.
莫诺苷调控糖尿病周围神经病变大鼠坐骨神经氧化应激损伤的机制
Mechanism of Morroniside regulating oxidative stress injury of sciatic nerve in diabetic peripheral neuropathy rats
摘要
Abstract
Objective To investigate the impacts of Morroniside on oxidative stress injury of sciatic nerve in diabetic pe-ripheral neuropathy(DPN)rats.Methods A total of 48 rats were grouped into a control group,DPN group,Morroniside group,Morroniside+Brusatol group,each group consisted of 12 rats.The changes in fasting blood glucose,mechanical pain threshold,thermal pain threshold,and sciatic nerve conduction velocity were detected.Transmission electron microscopy observe the ultrastructural changes of the sciatic nerve.ELISA method detect the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),malondialdehyde(MDA),and superoxide dismutase(SOD)activity in the sciatic nerve.West-ern blot detect nuclear factor erythroid 2 related factor 2(Nrf2),silent mating-type information regulation 2 homolog 1(SIRT1),and forkhead box class o3(Foxo3)proteins in the sciatic nerve.Results Sciatic nerve myelin in DPN group,Morro-niside group,Morroniside+Brusatol group were significantly damaged,fasting blood glucose,heat pain threshold,contents of IL-6,TNF-α and MDA in sciatic nerve were higher than those in control group,mechanical pain threshold,sensory and motor nerve conduction velocity,SOD activity in sciatic nerve and expression of Nrf2,SIRT1 and Foxo3 protein were lower than those in control group(P＜0.05).The ultrastructural damage of sciatic nerve tissue in Morroniside group was improved,and the contents of fasting blood glucose,heat pain threshold,IL-6,TNF-α and MDA in sciatic nerve were lower than those in DPN group.The mechanical pain threshold,sensory and motor nerve conduction velocity,SOD activity and Nrf2,SIRT1,Foxo3 protein expression in sciatic nerve were higher than those in DPN group(P＜0.05).The protective effect of Morroniside was reversed when Brusatol,an Nrf2 inhibitor,was given in addition to Morroniside group.Conclusion Morro-niside may improve oxidative stress injury of the sciatic nerve in DPN rats by activating the Nrf2/SIRT1/Foxo3 pathway.关键词
莫诺苷/糖尿病周围神经病变/核因子E2相关因子2/沉默信息调节因子2相关酶1/叉头转录因子o3/坐骨神经/氧化应激Key words
Morroniside/Diabetic peripheral neuropa-thy/Nuclear factor erythroid 2 related factor 2/Silent mat-ing-type information regulation 2 homolog 1/Forkhead box class o3/Sciatic nerve/Oxidative stress分类
医药卫生引用本文复制引用
邓小兰,那丽莎,于雪,张凯,曲娜..莫诺苷调控糖尿病周围神经病变大鼠坐骨神经氧化应激损伤的机制[J].中国当代医药,2024,31(18):4-8,5.基金项目
黑龙江省中医药管理局中医药科研课题(ZHY2022-042). （ZHY2022-042）
