吉林中医药2024，Vol.44Issue(7)：838-843,6.DOI:10.13463/j.cnki.jlzyy.2024.07.021
温振运气方对慢性疲劳综合征大鼠认知功能的影响
Effects of Wenzhen Yunqi formula on cognitive function in rats with chronic fatigue syndrome
摘要
Abstract
Objective To investigate the effects of Wenzhen Yunqi formula(WZYQ formula)on cognitive function in rats with chronic fatigue syndrome(CFS)and its possible mechanism of action.Methods Forty rats were divided into 10 each in the blank group,CFS model group,WZYQ formula group,and donepezil hydrochloride group,and a CFS model was established using the multiple chronic stress method in rats of all the groups except the blank group.After 30 days of modeling,1.039g/(kg·d)of Wenzhen Yunqi formula was given by gavage in the WZYQ formula group,and 0.51mg/(kg·d)of donepezil hydrochloride was given by gavage in the western medicine group,while 10ml/kg of saline was given by gavage in the blank group and the model group,daily for 4 continuous weeks.After that,behavioral tests were performed in the four groups.The levels of DA,ChAT,and GABA were detected by the enzyme-linked immunosorbent assay(ELISA)and the levels of pentraxin(5-HT)and neurogranulin(Ng)were detected by immunohistochemical staining after the behavioral tests.Results Compared with the model group,the positioning navigation latency,number of platform jumping error,and central compartment dwell time were decreased in both the Chinese medicine and western medicine groups(P<0.01 or P<0.05),while the platform jumping latency,number of platform crossing,platform quadrant swimming time,exhaustion swimming time,and number of hanging tail struggle were all increased in both the Chinese medicine and western medicine groups(P<0.01 or P<0.05),and the horizontal movement time,vertical movement time and frequency were increased in the Chinese medicine group compared with the model group(P<0.01),while the hanging tail rest time was decreased(P<0.01).The expression levels of DA,5-HT,GABA,ChAT and Ng were all increased in both the Chinese medicine group and the western medicine group(P<0.01 or P<0.05).Conclusion Wenzhen Yunqi formula can regulate the expression of central neurotransmitters,ChAT,and Ng,reduce somatic fatigue,alleviate negative emotions,and improve cognitive dysfunction in CFS rats.关键词
慢性疲劳综合征/认知功能/温振运气方/神经递质Key words
chronic fatigue syndrome(CFS)/cognitive function/Wenzhen Yunqi formula/neurotransmitters分类
医药卫生引用本文复制引用
李俊锋,张振贤,史佳宁,封鑫宇..温振运气方对慢性疲劳综合征大鼠认知功能的影响[J].吉林中医药,2024,44(7):838-843,6.基金项目
国家自然科学基金项目(82274430) （82274430）
上海中医药大学附属岳阳中西医结合医院科研项目(2021yygm03) （2021yygm03）
