江苏大学学报（医学版）2024，Vol.34Issue(4)：283-289,306,8.DOI:10.13312/j.issn.1671-7783.y230104
细胞焦亡相关蛋白在胎膜早破孕妇胎膜组织中的表达及其与妊娠结局的关系
Expression of pyroptosis related proteins in fetal membranes of pregnant women with premature rupture of membranes and its relationship with pregnancy outcome
钱云英 1钱桂英 2蔡奚梅 1顾伟群 1倪海燕 1朱丹婴 1徐英芳 1计文霞1
作者信息
- 1. 常熟市中医院妇产科,江苏常熟 215500
- 2. 常熟市中医院药剂科,江苏常熟 215500
- 折叠
摘要
Abstract
Objective:To explore the expression of pyroptosis related protein cysteinyl aspartate specific proteinase-1(Caspase-1)and nucleotide-binding oligomerization domain-like receptor protein-3(NLRP3)in fetal membranes of pregnant women with premature rupture of membranes(PROM)and their relationship with pregnancy outcomes.Methods:A total of 135 pregnant women with PROM who received medical treatment and delivered at the Obstetrics and Gynecology Department of Changshu Traditional Chinese Medicine Hospital from January 2020 to January 2023 were selected as the observation group,and other 135 healthy pregnant women who received prenatal examination and delivered during the same period were selected as the control group.The expression of NLRP3 mRNA and Caspase-1 mRNA in the fetal membrane tissue of the two groups of pregnant women was compared.One hundred and thirty-five pregnant women with PROM were divided into a poor pregnancy group(n=50)and a good pregnancy group(n=85)based on pregnancy outcomes.Independent risk factors for poor pregnancy in pregnant women with PROM were analyzed using univariate and multivariate Logistic analysis.Logistic regression model and restricted cubic splines(RCS)were used to analyze the dose-response relationship between the expression of pyroptosis related proteins in fetal membranes of pregnant women with PROM and adverse pregnancy.A nomogram prediction model was constructed by using independent influencing factors and the model was also verified.Results:The expression of NLRP3 mRNA and Caspase-1 mRNA in the fetal membrane tissue of the observation group was significantly higher than that of the control group(P＜0.001).Visit time,reproductive tract infections,and the mRNA expression levels of NLRP3 and Caspase-1 are independent risk factors for poor pregnancy outcomes in pregnant women with PROM(P＜0.05).The RCS results showed that the expression levels of NLRP3 and Caspase-1 in the fetal membrane tissue of pregnant women with PROM showed a non-linear dose-response relationship with adverse pregnancy outcomes.The highest risk of adverse pregnancy outcomes occurred at NLRP3 mRNA expression levels of 1.20(OR=1.818,95％CI:1.673-1.932)and Caspase-1 mRNA expression levels of 1.25(OR=2.735,95％CI:1.132-3.821).The constructed nomogram prediction model has good discrimination,accuracy,and clinical applicability.Conclusion:Caspase-1 mRNA and NLRP3 mRNA are highly expressed in the fetal membrane tissue of pregnant women with PROM,and they are independent risk factors for adverse pregnancy outcomes in pregnant women with PROM.With the increase of expression levels,the risk of adverse pregnancy in pregnant women with PROM also increases.关键词
细胞焦亡相关蛋白/半胱氨酸天冬氨酸特异性蛋白酶-1/核苷酸结合寡聚化结构域样受体蛋白3/胎膜早破/胎膜组织/妊娠结局Key words
pyroptosis related protein/cysteinyl aspartate specific proteinase(Caspase-1)/nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)/premature rupture of membranes(PROM)/fetal membrane tissue/pregnancy outcome分类
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钱云英,钱桂英,蔡奚梅,顾伟群,倪海燕,朱丹婴,徐英芳,计文霞..细胞焦亡相关蛋白在胎膜早破孕妇胎膜组织中的表达及其与妊娠结局的关系[J].江苏大学学报（医学版）,2024,34(4):283-289,306,8.
