扬州大学学报（农业与生命科学版）2024，Vol.45Issue(3)：17-27,11.DOI:10.16872/j.cnki.1671-4652.2024.03.002
2C类蛋白磷酸酶基因OsPP2C49调控水稻干旱和高温胁迫响应的机制研究
Mechanism of the rice type 2C protein phosphatase gene OsPP2C49 in regulating drought and high-temperature stress responses
摘要
Abstract
Rice is a crucial food crop,and its yield is significantly affected by adverse environmental conditions,inclu-ding drought,high-temperature,and salinity.ABA,a stress-responsive hormone,plays a crucial role in regulating sever-al adversity stress responses.In this study,we focused on the ABA signaling component,OsPP2C49,a member of the clade A protein phosphatase 2C family and found that OsPP2C49 negatively regulated drought and high temperature stress responses.Knockout of OsPP2C49 significantly improved the survival rate of rice under drought and high-temperature conditions,whereas overexpression of OsPP2C49 resulted in a significant decrease in survival rate.Notably,the expres-sion of OsPP2C49 was induced by various adversity stresses.Furthermore,OsbZIP10,OsbZIP40,and OsbZIP42 tran-scription factors binding to the OsPP2C49 promoter,thereby activating its transcription.Under drought and high-tem-perature conditions,the expression of these bZIP transcription factors was also significantly induced,suggesting that the stress-induced expression of OsPP2C49 was likely to be mediated by different bZIP transcription factors.The results will be useful for breeding rice varieties that are resistant to drought and high-temperature.关键词
水稻/脱落酸/2C类蛋白磷酸酶/OsPP2C49/干旱胁迫/高温胁迫Key words
rice/abscisic acid/type 2C protein phosphatase/OsPP2C49/drought tolerance/high-temperature tolerance分类
农业科技引用本文复制引用
朱子杰,卜柳冰,王雨鑫,徐佳婷,刘丹彤,缪军..2C类蛋白磷酸酶基因OsPP2C49调控水稻干旱和高温胁迫响应的机制研究[J].扬州大学学报（农业与生命科学版）,2024,45(3):17-27,11.基金项目
国家自然科学基金青年基金项目(32100259) （32100259）
江苏省大学生创新创业训练计划项目(202211117048Z) （202211117048Z）
