扬州大学学报（农业与生命科学版）2024，Vol.45Issue(3)：64-71,8.DOI:10.16872/j.cnki.1671-4652.2024.03.007
基于NLRP-3炎症小体探讨附子理中丸对伊立替康诱导的大鼠腹泻抗炎止泻作用
Role of Fuzi Lizhong pill anti-inflammatory and antidiarrheal effects on rats with irinotecan-induced diarrheal based on NLRP-3 inflammasomes
摘要
Abstract
To investigate the anti-inflammatory and antidiarrheal effects of Fuzi Lizhong pill and to study its mecha-nisms through NLRP-3 inflammasomes.Wistar rats were divided into control group,model group,berberine group,IKK-β inhibitor group,Fuzi Lizhong pill low,middle and high dose groups(10,20,40 g·kg-1 respectively).A rat model of irinotecan-induced diarrhea was established in the model group,berberine group,IKK-β inhibitor group,Fuzi Lizhong pill low,middle and high dose groups.After successful modeling,except for the model group,each group was given the corresponding drug for gastric gavage,and the control group was given distilled water of equal volume,and the gastric gavage was continuously gavaged for 30 days and then to detect relevant indexes.The results showed that in the model group the histopathological changes of the colon in the model group and its ultrastructural damage were serious,moreover,the levels of IL-1β,IL-18 and TNF-a,the relative expressions of mRNA of NLRP-3,Caspase-1,ASC,GSDMD and the relative expressions of NLRP-3,Caspase-1 and ASC proteins all significantly increased compared to the control group.However,berberine group,IKK-β inhibitor group,Fuzi Lizhong pill low,middle and high dose groups can im-prove above indexes in different degrees compared to the model group.It is supposed that Fuzi Lizhong pill can alleviate inflammation and diarrhea by inhibiting the activation of NLRP-3 inflammasomes.关键词
附子理中丸/腹泻/NLRP-3炎症小体/炎症Key words
Fuzi Lizhong pill/diarrhea/NLRP-3 inflammasomes/inflammation分类
农业科技引用本文复制引用
罗菲,郭建宏,窦玉玉,覃洪含,唐汉庆,李嘉慧,梁凌玲,潘斌,黄永毅..基于NLRP-3炎症小体探讨附子理中丸对伊立替康诱导的大鼠腹泻抗炎止泻作用[J].扬州大学学报（农业与生命科学版）,2024,45(3):64-71,8.基金项目
广西自然科学基金面上项目(2020GXNSFAA297159) （2020GXNSFAA297159）
广西百色市区域多发病联合专项(百科20224127) （百科20224127）
