扬州大学学报（农业与生命科学版）2024，Vol.45Issue(3)：72-81,10.DOI:10.16872/j.cnki.1671-4652.2024.03.008
鼠抗人DCP单克隆抗体的类型转换与表达
Class switching and expression of a mouse anti-human DCP antibody
摘要
Abstract
In this study,we used a recently-prepared mouse anti-human DCP monoclonal antibody,7C2,of IgM class as a research object to establish a system for class switching and expression of antibody.The variable regions of light and heavy chains of 7C2 antibody were cloned from hybridoma cells.Then,we fused the variable region sequences of 7C2 with the constant region sequences of mouse IgG1 antibody into a eukaryotic expression vector and transferred the recombinant plasmid into 293 cell line.Western blot analysis showed that recombinant IgG1 antibody was successfully expressed in the cells and secreted into culture supernatant.After further optimizing the factors affecting the expression efficiency of anti-bodies,such as codons,host cells and signal peptides,we established a double-plasmid transient expression system and a bicistronic expression system,which can be used to convert murine IgM into IgG antibodies and express them.This study provides an important way to expand the application of non-IgG type antibodies and also a reference for improving the ex-pression efficiency of recombinant antibodies in eukaryotic system.关键词
单克隆抗体/基因工程/抗体类型转换/表达条件优化Key words
monoclonal antibody/genetic engineering/antibody class switch/optimization of expression conditions分类
农业科技引用本文复制引用
廖凯,张智萍,丁笠,张新跃..鼠抗人DCP单克隆抗体的类型转换与表达[J].扬州大学学报（农业与生命科学版）,2024,45(3):72-81,10.基金项目
江苏省研究生科研创新计划项目(KYCX21-3206) （KYCX21-3206）
