江西农业学报2024，Vol.36Issue(6)：42-54,13.DOI:10.19386/j.cnki.jxnyxb.2024.06.007
杉木林下不同生长年限灵芝栽培土壤微生物群落变化特征
Changes of Soil Microbial Communities in Ganoderma lucidum Cultivation with Different Growth Years under Fir Forests
摘要
Abstract
In order to study the changes of Ganoderma lucidum with different growth years on the structure and diversity of soil microbial communities under fir forests,the correlation between soil factors and fungal and bacterial communities was preliminarily explored.Illumina MiSeq high-throughput sequencing technology was used to sequence the amplicon sequences of Ganoderma lucidum cultivation soil cultivated under fir forest for 1 year(S1),2 years(S2),3 years(S3)and 4 years(S4),and the characteristics of microbial community changes in Ganoderma lucidum cultivation soil under fir forest were analyzed.The results showed that at the level of the phylum,the main dominant bacterial floras are Proteobaceria,Acidobacteriota,Actinobacterota,and Chloroflexi,among which the advantages of Proteobaceria and Acidobacteria are more significant.The dominant fungal floras were Ascomycota,Basidiomycota,Mortierellomycota,Rozellomycota and unclassified fungi,among which the Ascomycota dominated the four groups of samples,and the relative abundance accounted for the highest proportion.At the genus level,the relative abundance of bacterial norank_f_Xanthobacteraceae and norank_f__norank_o__Elsterales genus was relatively high in the four groups of samples,and there was no significant difference in the proportion of relative abundance for four years.At the level of the genus of fungi,the dominant flora differs from year to year,and the S1 dominant flora is Trichoderma.The main dominant flora of S2 is Hypochnicium.The main dominant flora of S3 is unclassified_k__Fungi,and the dominant flora of S4 is the genus unclassified_p_Basidiomycota.Soil total carbon,total phosphorus and laccase were the key factors affecting the structure of soil microbial community and soil microbial diversity in Ganoderma lucidum cultivation under fir forests at different growth years.关键词
不同生长年限灵芝/杉木林下灵芝栽培/土壤微生物/土壤养分Key words
Ganoderma lucidum with different growth years/Ganoderma lucidum cultivation under fir forest/Soil microorganism/Soil nutrient分类
农业科技引用本文复制引用
梁晓君,周春柳,韦仕岩,杨再锋,何建,吴圣进,祁亮亮,王晓国..杉木林下不同生长年限灵芝栽培土壤微生物群落变化特征[J].江西农业学报,2024,36(6):42-54,13.基金项目
广西重点研发项目(桂科AB22035030) （桂科AB22035030）
国家重点研发项目(2022YFD1100104) （2022YFD1100104）
广西农科院基本业务专项(桂农科2023YM07、桂农科2021YT094) （桂农科2023YM07、桂农科2021YT094）
国家现代农业产业技术体系广西食用菌创新团队建设专项(Nycytxgx-cxtd-2021-07-02) （Nycytxgx-cxtd-2021-07-02）
国家现代农业产业技术体系建设项目(CARS-20). （CARS-20）
