解放军医学院学报2024，Vol.45Issue(5)：457-462,480,7.DOI:10.12435/j.issn.2095-5227.2024.033
宏基因组二代测序技术对结核性与非结核性脊柱感染疾病的诊断价值研究
Diagnostic value of metagenomic next-generation sequencing technology for tuberculous and nontuberculous spinal infectious diseases
摘要
Abstract
Background Tuberculous spinal infection is a difficult disease in spinal surgery,and most of its early clinical symptoms are atypical,and the specificity of laboratory tests,imaging tests and histopathologic examinations is not high.Pathogenetic diagnosis is the gold standard for the diagnosis of tuberculous spinal infection,but the culture of pathogenic microorganisms has the disadvantages of long period and low positive rate.Metagenomic next-generation sequencing technology is currently used in clinical diagnosis,but there are fewer relevant literature reports on the comparison between mNGS and other common clinical detection methods.Objective To investigate the diagnostic value of metagenomic next-generation sequencing(mNGS)technology for tuberculous spinal infection disease.Methods Patients who were admitted to the Department of Spine Surgery of the Eighth Medical Center of Chinese PLA General Hospital and diagnosed as spine infection from February 2021 to September 2023 were included in this study.Specimens of blood,pus,and lesion tissue were collected for serological testing,bacterial culture,tuberculosis culture,pathological examination,X-pert,and mNGS testing,and the results were compared with clinical diagnosis(gold standard).The diagnostic value of different detection methods was analyzed.Results There were 68 males and 44 females included in this study,with age ranged from 14 years to 87 years(58.98±13.70 years).The clinical diagnosis revealed 63 cases of non-tuberculous spinal infection and 49 cases of spinal tuberculosis infection.mNGS detected positive results for spinal infection in 90 cases(80.4％),while microbial culture detected positive results in 51 cases(45.5％).In terms of determining tuberculosis infection in spinal infections,mNGS detected positive results in 34 cases(69.39％),while microbial culture detected positive results in only 17 cases(34.69％).Among the clinical diagnostic efficacy analysis results of mNGS,microbial culture,T-spot,and X-pert for spinal tuberculosis,mNGS showed the highest efficacy(AUC=0.839)with the highest accuracy in diagnosis,surpassing currently used clinical methods.The combined diagnostic approach of mNGS and the four methods showed the highest diagnostic efficacy,with ROC-AUC(95％CI)values of 0.846(0.701-0.971)and 0.876(0.773-0.962)respectively,significantly higher than the other three individual methods,with a Delong test P＜0.05.Conclusion mNGS enables rapid and efficient detection of pathogenic microorganisms in infectious diseases of the spine,and it has a high detection rate,sensitivity,and specificity for tuberculous spinal infections.This technology is a good and fast method for diagnosing and differentiating infectious diseases of the spine,providing guidance for the diagnosis of tuberculous spinal infections.关键词
脊柱感染/脊柱结核/宏基因二代测序/病原微生物/诊断Key words
spinal infection/spinal tuberculosis/metagenomic next-generation sequencing/pathogenic microorganisms/diagnosis分类
医药卫生引用本文复制引用
郭路明,崔旭,于龙,李力韬,吴云峰,李大伟,鲍达,罗展鹏,刘宁,杨尚杰..宏基因组二代测序技术对结核性与非结核性脊柱感染疾病的诊断价值研究[J].解放军医学院学报,2024,45(5):457-462,480,7.基金项目
国家自然科学基金面上项目(81972079) （81972079）
