辣椒素敏感神经元对小鼠牙周炎骨吸收影响的研究OACSTPCD

Background Periodontitis is an infectious disease initiated by plaque.However,the role of peripheral nerves in the progression of periodontitis remains unclear.Objective To study the effect of capsaicin-sensitive neurons on the progression of experimental periodontitis in mice.Methods Twenty male C57BL/6 mice were randomly divided into ablation group(Cap)and non-ablation group(Veh),10 mice in each group.The Cap group received injections of high-concentration capsaicin(5 μg/mL)into the ocular/maxillary region of bilateral trigeminal nerves to ablate capsaicin-sensitive neurons,while the Veh group received injections of a vehicle containing 1％DMSO and 10％Tween-80 phosphate-buffered saline.One week later,a ligature was tied around the left maxillary second molar of each mouse,the right maxillary second molar was untreated as a control.The mice were then divided into four groups:non-ablative control(Veh/Con),ablative control(Cap/Con),non-ablative periodontitis(Veh/Lig),and ablative periodontitis(Cap/Lig).One week later,the maxilla was examined by micro-CT to observe the reduction of alveolar bone.After decalcification,the HE staining and the TRAP staining were performed to observe soft and hard tissue destruction and osteoclast formation.Quantitative real-time polymerase chain reaction(RT-qPCR)was employed to detect expression levels of inflammatory factors TNF-α and IL-1 β in gum tissue.Results The results of micro-CT showed that compared with the Veh/Lig group,alveolar bone resorption was decreased in the Cap/Lig group(P＜0.05).HE staining and TRAP staining showed that compared with the Veh/Lig group,damage to soft and hard tissues was reduced in the Cap/Lig group;furthermore,there was also a decrease in the number of osteoclasts around the alveolar bone(P＜0.05).RT-qPCR results indicated that expression levels of inflammatory factors TNF-αand IL-1β were lower in the Cap/Lig group than those in the Veh/Lig group(P＜0.05).Conclusion Capsaicin-sensitive neurons can promote bone resorption in periodontitis,which may be related to osteoclast differentiation and local tissue inflammation.