检验医学与临床2024，Vol.21Issue(14)：2075-2081,7.DOI:10.3969/j.issn.1672-9455.2024.14.019
LncRNA CBR3-AS1 调节 miR-448/TFAM 轴对结直肠癌细胞恶性生物学行为的影响
Impact of LncRNA CBR3-AS1 regulating miR-448/TFAM axis on malignant biological behavior of colorectal cancer cells
摘要
Abstract
Objective To investigate the impact of long non-coding RNA(LncRNA)CBR3-AS1 on the malignant biological behavior of colorectal cancer(CRC)cells and its mechanism.Methods A total of 40 pairs of CRC tissue samples and adjacent paracancerous tissue samples were collected from the Jinan Munici-pal Fifth People's Hospital from January 2021 to June 2022.The real-time fluorescence quantitative polymer-ase reaction(qRT-PCR)was applied to detect the expressions of LncRNA CBR3-AS1,microRNA-448(miR-448)and mitochondrial transcription factor A(TFAM)mRNA in human CRC tissue,paracancerous tissues,CRC cell line and normal colorectal epithelial cells.According to the expression levels of LncRNA CBR3-AS1 in different CRC cell lines,the cell lines used in subsequent experiments were screened.The targeting relation-ship between miR-448 with LncRNA CBR3-AS1 and TFAM was verified.Small interfering RNA(si-CBR3-AS1)targeting LncRNA CBR3-AS1 and miR-448 inhibitor were transfected into target cells,qRT-PCR and Western blot were applied to detect the mRNA and protein expression of LncRNA CBR3-AS1,miR-448 and TFAM;the cell viability,apoptosis rate,migration and invasion ability were detected by CCK-8 assay,flow cy-tometry,scratch healing assay and Transwell assay,respectively.Results LncRNA CBR3-AS1 and TFAM mRNA were highly expressed in CRC tissues and cells,while miR-448 was lowly expressed.In addition,the expression of LncRNA CBR3-AS1 was the highest in HCT116 cells,so HCT116 cells were selected for subse-quent targeting validation and transfection experiments.After verification,miR-448 had a targeted relationship with LncRNA CBR3-AS1 and TFAM in HCT116 cells.Transfecting si-CBR3-AS1 could reduce the expression levels of LncRNA CBR3-AS1,and TFAM mRNA and protein in HCT116 cells,meanwhile reduce the cell via-bility,scratch healing rate,number of migrated and invasive cells,and increase the miR-448 expression level and apoptosis rate;transfecting miR-448 inhibitor on the basis of trassfecting si-CBR3-AS1 could weaken the effect of interfering LncRNA CBR3-AS1 expression on HCT116 cells.Conclusion Interfering LncRNA CBR3-AS1 could inhibit the malignant biological behavior of CRC cells,and its mechanism may be related to targeting the miR-448/TFAM axis.关键词
结直肠癌/长链非编码RNA CBR3-AS1/微小RNA-448/线粒体转录因子A/迁移/侵袭Key words
colorectal cancer/long non-coding RNA CBR3-AS1/microRNA-448/mitochondrial transcription factor A/migration/invasion分类
医药卫生引用本文复制引用
徐娜娜,胡敬暖,王新玮..LncRNA CBR3-AS1 调节 miR-448/TFAM 轴对结直肠癌细胞恶性生物学行为的影响[J].检验医学与临床,2024,21(14):2075-2081,7.基金项目
山东省济南市科技局计划项目(201221027). （201221027）
