检验医学与临床2024，Vol.21Issue(14)：2089-2093,5.DOI:10.3969/j.issn.1672-9455.2024.14.021
血清TLR4、TIMP-1水平与小儿热性惊厥临床特征的关系及对继发癫痫的预测价值
Relationship between serum TLR4 and TIMP-1 levels with clinical characteristics of pediatric febrile convulsion and its predictive value for secondary epilepsy
摘要
Abstract
Objective To analyze the relationship between the levels of serum Toll-like receptor 4(TLR4)and tissue inhibitor of matrix metalloproteinase 1(TIMP-1)with the clinical characteristics of febrile convul-sion(FC)in children and its predictive value for secondary epilepsy.Methods A total of 320 children patients with FC from January 2019 to June 2022 were selected as the study group,other 150 children with fever and no convulsions during the same period were selected as the fever group,while 50 healthy children undergoing physical examination served as the control group.The FC children patients were divided into the epilepsy group and non-epilepsy group according to whether or not having secondary epilepsy.The enzyme linked im-munosorbent assay was applied to detect serum TLR4 and TIMP-1 levels.The Pearson correlation was applied to analyze the correlation between the levels of TLR4 and TIMP-1 with the clinical indexes.The receiver oper-ating characteristic(ROC)curve was applied to analyze the value of serum TLR4 and TIMP-1 in predicting FC secondary epilepsy.The Logistic regression was applied to analyze the influencing factors of secondary epi-lepsy in the children patients with FC.Results The serum TLR4 and TIMP-1 levels in children patients with FC,febrile children without convulsion and healthy children were in turn decreased,and pairwise comparison showed statistically significant differences(P＜0.05).There were statistically significant differences in the perinatal abnormalities,TNF-α,CRP,IL-1β levels and AEEG score between the study group and fever group(P＜0.05).The serum TLR4 and TIMP-1 levels in the epilepsy group were significantly higher than those in the non-epilepsy group(P＜0.05).There were statistically significant differences in the number of first con-vulsion,convulsion duration,fever duration before the first seizure,perinatal abnormalities,TNF-α,CRP,IL-1βlevels and AEEG scores between the epilepsy group and non-epilepsy group(P＜0.05).There was a positive correlation between serum TLR4 level and TIMP-1 level(P＜0.05);the serum TLR4 and TIMP-1 levels were positively correlated with TNF-α,CRP and IL-1β(P＜0.05),and negatively correlated with the AEEG score(P＜0.05).The area under the curve(AUC)of TLR4 and TIMP-1 combined detection in predicting secondary epilepsy in children with FC was obviously higher than AUC of single item detection(ZTLR4-combination=3.016,P=0.003,ZTIMP-1-combination=2.232 P=0.026).The Logistic regression analysis results showed that the TLR4,TIMP-1,TNF-α,CRP and IL-1β levels increase and AEEG score decrease were the risk factors for the secondary epilepsy in children patients with FC(P＜0.05).Conclusion The serum TLR4 and TIMP-1 levels are closely correlated to the clinical characteristics in children patients with FC.TLR4 and TIMP-1 might be the influencing factors of FC secondary epilepsy.关键词
Toll样受体4/基质金属蛋白酶组织抑制剂1/小儿热性惊厥/癫痫/相关性Key words
Toll-like receptor 4/tissue inhibitor of matrix metalloproteinase-1/infantile febrile con-vulsion/epilepsy/correlation分类
医药卫生引用本文复制引用
张润春,李树华,王玉珍,张静,曹志伟..血清TLR4、TIMP-1水平与小儿热性惊厥临床特征的关系及对继发癫痫的预测价值[J].检验医学与临床,2024,21(14):2089-2093,5.基金项目
河北省医学科学研究课题(20221747). （20221747）
