检验医学与临床2024，Vol.21Issue(14)：2131-2134,2144,5.DOI:10.3969/j.issn.1672-9455.2024.14.030
宏基因二代测序技术在社区获得性肺炎病原体检测中的应用进展
Progress in application of macrogene second-generation sequencing technology in detection of community-acquired pneumonia pathogens
摘要
Abstract
Community-acquired pneumonia(CAP)is one of the common infectious diseases.Its epidemio-logical characteristics vary with host and immune status,and the pathogen spectrum also shifts with time and space distribution.Accurate and rapid identification of pathogens and precise treatment are the key to improve the cure rate of the disease.The conventional detection methods include the cell culture,antibody and antigen detection,polymerase chain reaction(PCR),etc.,but they have the defects of slow recognition and low detec-tion rate,thus delay the opportunity of clinical treatment.The metagene second-generation sequencing tech-nology(mNGS)mainly sequences all microorganisms in samples through nucleic acid extraction,PCR ampli-fication,library construction and other steps,and identifies the pathogens by a large number of biological anal-ysis.mNGS is different from routine examination methods,has the advantages of rapid identification,all-round coverage and high sensitivity,and is gradually selected to be used in clinical diagnosis.Nowadays,mNGS has played an increasingly important role in the diagnosis and treatment of CAP,especially in the detection of some rare and new type pathogenic microorganisms,and it also has certain advantages in the detection of pathogens in special populations.However,as a new detection method,mNGS still has some shortcomings.For example,there is no uniform standard for the interpretation of test results,the results are greatly influ-enced by the patient's genetic material,and the test cost is high.In the future,the technique can be optimized by improving the pretreatment of pre-testing samples,reducing the cost of testing and simplifying the inter-pretation of the results after testing,so as to comprehensively promote the clinical application of mNGS.关键词
社区获得性肺炎/宏基因二代测序技术/聚合酶链反应/临床应用/病原体Key words
community-acquired pneumonia/macrogene second-generation sequencing technology/polymerase chain reaction/clinical application/pathogen分类
医药卫生引用本文复制引用
廖毓香,朱水泉,伍桂雄..宏基因二代测序技术在社区获得性肺炎病原体检测中的应用进展[J].检验医学与临床,2024,21(14):2131-2134,2144,5.基金项目
广西壮族自治区梧州市科技局基金项目(2021F01882). （2021F01882）
