临床口腔医学杂志2024，Vol.40Issue(7)：387-392,6.DOI:10.3969/j.issn.1003-1634.2024.07.002
miR-2355-3p通过靶向SERPINA3增强口腔鳞状细胞癌细胞的放射敏感性
MiR-2355-3p enhances radiosensitivity of oral squamous cell carcinoma cells by targeting SERPINA3
摘要
Abstract
Objective:To investigate the effect and mechanism of miR-2355-3p on radiosensitivity of oral squamous cell carcinoma(OSCC).Methods:Retrospectively collect 20 cases of OSCC and 20 cases of normal oral mucosal tissue.The miR-2355-3p expression was detected using qRT-PCR,and the clinical pathological characteristics of patients were analyzed.Using TCGA data to analyze the expression,prognosis and diagnostic value of miR-2355-3p in OSCC.qRT-PCR detected the relative expression of miR-2355-3p and serpin family A member 3(SERPINA3)in FADU cells,CCK-8,EdU,and flow cytom-etry were used to detect the cell viability,proliferation,and cell apoptosis.TargetScan 8.0 predicted the potential downstream target genes and binding sites of miR-2355-3p.Dual-luciferase reporter assay validated the correlation between miR-2355-3p and SERPINA3.qRT-PCR and Western blot detected the relative expression of SERPINA3 mRNA and protein in FADU cells.Results:miR-2355-3p was significantly upregulated in OSCC,indicating a prognosis for OSCC patients with high levels of miR-2355-3p.The AUC of miR-2355-3p in predicting OSCC was 0.864(95％CI:0.798～0.931)for OSCC.Inhibition of miR-2355-3p led to downregulation of miR-2355-3p expression in FADU cells.Ionizing radiation(IR)treatment suppressed cell activity and proliferation while stimulated cell apoptosis of FADU cells.Transfection with miR-2355-3p inhibitor further inhibited cell viability and proliferation,while increased apoptosis of FADU cells.miR-2355-3p directly targeted SERPINA3.Inhibition of miR-2355-3p promoted the expression of SERPINA3 mRNA and protein in FADU cells.Conclusion:miR-2355-3p enhanced the radiosensitivity of oral squamous cell carcinoma cells by targeting SERPINA3.关键词
口腔鳞状细胞癌/miR-2355-3p/SERPINA3/细胞增殖/放射敏感性Key words
Oral squamous cell carcinoma/miR-2355-3p/SERPINA3/Cell proliferation/Radiosensitivity分类
医药卫生引用本文复制引用
陆开睿,潘树矿,胡梦甜,丁凤,王育新,薛洪宝..miR-2355-3p通过靶向SERPINA3增强口腔鳞状细胞癌细胞的放射敏感性[J].临床口腔医学杂志,2024,40(7):387-392,6.基金项目
安徽省高校学科(专业)拔尖人才学术资助项目(gxbjZD2022041) （专业）
安徽省新时代育人质量工程研究生教育项目(2022lhpysfjd061、2022sshqygzz033) （2022lhpysfjd061、2022sshqygzz033）
