首页|期刊导航|西南医科大学学报|羟基红花黄色素A抑制血管紧张素Ⅱ介导的心脏成纤维细胞肌化

羟基红花黄色素A抑制血管紧张素Ⅱ介导的心脏成纤维细胞肌化

蒋洁王立群刘雪茹杨艳谭晓秋陈唐葶

西南医科大学学报2024，Vol.47Issue(4)：306-310,5.

西南医科大学学报2024，Vol.47Issue(4)：306-310,5.DOI:10.3969/j.issn.2096-3351.2024.04.006

羟基红花黄色素A抑制血管紧张素Ⅱ介导的心脏成纤维细胞肌化

Inhibition of angiotensin Ⅱ-mediated cardiac fibroblast myofibroblast transformation by hydroxy crocetin A

蒋洁 ¹王立群 ²刘雪茹 ³杨艳 ¹谭晓秋 ⁴陈唐葶¹

作者信息

1. 西南医科大学医学电生理学教育部重点实验室,医学电生理四川省重点实验室,心血管医学研究所(泸州 646000)
2. 西南医科大学教育部代谢性心血管疾病医药基础研究创新中心(泸州 646000)
3. 西南医科大学附属医院麻醉科(泸州 646000)
4. 西南医科大学医学电生理学教育部重点实验室,医学电生理四川省重点实验室,心血管医学研究所(泸州 646000)||西南医科大学基础医学院(泸州 646000)
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摘要

Abstract

Objective To study the effects of Hydroxysafflor yellow A(HSYA)on angiotensin Ⅱ(Ang-Ⅱ)-mediated myocar-dial fibrosis.Methods HFC-aa cells were randomly divided into the control group,the Ang-Ⅱ group,the Ang-Ⅱ+HSYA(5µM)group,the Ang-Ⅱ+HSYA(25µM)group,the Ang-Ⅱ+HSYA(50µM)group,and the Ang-Ⅱ+HSYA(100µM)group.Cells in the Ang-Ⅱ group were treated with 0.5µM Ang-Ⅱfor 24 hours,and cells in the Ang-Ⅱ+HSYA groups were treated with HSYA(5,25,50,and 100µM)and Ang-Ⅱ(0.5µM)for 24 hours.Cells in the control group were treated with the same volume of PBS.Cell wound healing and transwell migration assays were used to determine the cell migration.DCFH-DA fluorescent probe was utilized to de-tect the intracellular ROS level,and western blot was used to evaluate the protein expression of α-SMA,Collagen Ⅰ,Collagen Ⅲ and TGF-β1.Results Ang-Ⅱ could increase the migration and ROS production of cardiac fibroblasts.HSYA inhibited Ang-Ⅱ-mediated cardiac fibroblasts migration and ROS production.Western blot showed that HSYA inhibited Ang-Ⅱ-mediated protein expression of α-SMA,Collagen Ⅰ,Collagen Ⅲ and TGF-β1 in cardiac fibroblasts,and the difference was statistically significant(P<0.05).Con-clusion HSYA inhibited the differentiation of cardiac fibroblasts into myofibroblasts induced by Ang-Ⅱ by reducing the production of reactive oxygen species(ROS)and down-regulating the TGF-β1 signaling pathway.

关键词

心肌纤维化/羟基红花黄色素A/血管紧张素Ⅱ/活性氧/转化生长因子β1

Key words

Myocardial fibrosis/Hydroxysafflor yellow A/Angiotensin-Ⅱ/Reactive oxygen species(ROS)/Transforming growth factor-β1(TGF-β1)

分类

医药卫生

引用本文复制引用

蒋洁,王立群,刘雪茹,杨艳,谭晓秋,陈唐葶..羟基红花黄色素A抑制血管紧张素Ⅱ介导的心脏成纤维细胞肌化[J].西南医科大学学报,2024,47(4):306-310,5.

基金项目

国家自然科学基金(81900277) （81900277）

四川省科技计划联合创新专项(2022YFS0607,2022YFS0632,2022YFS0630) （2022YFS0607,2022YFS0632,2022YFS0630）

泸州市科技计划项目(2023JYJ004) （2023JYJ004）

西南医科大学学报

ISSN：2096-3351

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