西南医科大学学报2024，Vol.47Issue(4)：311-315,5.DOI:10.3969/j.issn.2096-3351.2024.04.007
迷迭香酸对野百合碱诱导肺动脉高压大鼠的作用及机制
Effect and mechanism of rosmarinic acid on pulmonary arterial hypertension induced by monocrotaline in rats
摘要
Abstract
Objective To investigate the effect of rosmarinic acid(RosA)on monocrotaline(MCT)-induced pulmonary arterial hypertension(PAH)in rats and its mechanism.Methods Thirty male SD rats were randomly divided into control group(n=10),PAH model group(n=10)and rosmarinic acid treatment group(n=10).The PAH model was established by single subcutaneous injection of MCT(60 mg/kg).The control group and MCT group were given 0.9%stroke-physiological saline solution by intraperitoneal injection every day,and the RosA group was given rosmarinic acid(20 mg/kg)dissolved in 0.9%stroke-physiological saline solution by subcuta-neous injection.After 21 days,the mean pulmonary arterial pressure(mPAP)and right ventricular hypertrophy index(RVHI)of the rats were detected.Pulmonary arteriole remodeling was measured by HE staining.The expression levels of malondialdehyde(MDA),su-peroxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in lung tissues were detected by Elisa.The expression of α-smooth muscle actin(α-SMA)and proliferating cell nuclear antigen(PCNA)in lung tissue was detected by immunohistochemistry.Results Compared with MCT group,the rats in MCT+RosA group exhibited significant reductions in mPAP and RVHI,pulmonary artery remod-eling and pulmonary vascular fibrosis were decreased,the expression levels of α-SMA and PCNA in pulmonary arteriole were de-creased,and the expression level of SOD in lung tissue was significantly increased.The expression level of MDA was significantly de-creased(P<0.05).Conclusion RA could reduce the mPAP,improve right ventricular hypertrophy and pulmonary vascular remodel-ing in MCT-induced PAH rats,which might be associated with inhibition of lung tissue oxidative stress and the expression levels of α-SMA and PCNA in pulmonary vascular.关键词
迷迭香酸/肺动脉高压/肺血管重构/右心室肥厚Key words
Rosmarinic acid/Pulmonary artertension hypertension/Pulmonary vascular remodeling/Right ventricular hypertro-phy分类
医药卫生引用本文复制引用
陈秋宏,杨红,郑琴,郭婧仪,王昱媛,吴奇..迷迭香酸对野百合碱诱导肺动脉高压大鼠的作用及机制[J].西南医科大学学报,2024,47(4):311-315,5.基金项目
成都市科技局技术创新研发项目(2022-YF05-01459-SN) （2022-YF05-01459-SN）
四川省中医药管理局科研项目(2023MS177) （2023MS177）
成都医学院研究生创新基金(YCX2022-01-23) （YCX2022-01-23）
成都市市级医学重点专科项目(CDS2022Z076) （CDS2022Z076）
