Gh_D11G050000参与棉花黄萎病抗性的功能分析OA北大核心CSTPCD

[Objective]This study aims to provide a theoretical basis for molecular breeding of disease-resistant cotton by cloning Gh_D11G050000 gene,and to analyze its function and mechanisms in cotton resistance to Verticillium wilt.[Methods]Bioinformatic method was utilized to analyze the sequence characteristics and phylogenetic relationship of Gh_D11G050000.Quantitative real-time polymerase chain reaction(qRT-PCR)was employed to investigate the expression pattern of this gene,and it's expression level changes after infected by Verticillium dahliae.The function of this gene was validated using virus-induced gene silencing(VIGS)technique.Transcriptome sequencing analysis and the detection of relative expression levels of related genes were conducted to explore the mechanisms of disease resistance.[Results]Gh_D11G050000 exhibited close relationship with Gh_A11G049600 and Gr_11G034620 protein.qRT-PCR analysis revealed that Gh_D11G050000 was highly expressed in cotton roots,and the expression level of Gh_D11G05000 was significantly increased after V.dahliae infection.Gh_D11G050000 silenced cotton plants showed reduced resistance against V.dahliae,characterized by aggravated browning of stem vascular bundles,increased the number of stem segments with bacterial propagation,as well as significantly increased the rate of diseased plants and disease index.Transcriptome analysis combined with qRT-PCR demonstrated that Gh_D11G050000 silenced cotton plants showed decreased transcription levels for several genes in jasmonic acid(JA)signaling pathway,ethylene(ET)signaling pathway,and lignin synthesis pathway.[Conclusion]Gh_D11G050000 positively regulates cotton resistance to Verticillium wilt by influencing the expression of several genes involved in lignin synthesis,JA,and ET signaling pathways.