棉花学报2024，Vol.36Issue(3)：211-224,14.DOI:10.11963/cs20240027
Gh_D11G050000参与棉花黄萎病抗性的功能分析
Functional analysis of Gh_D11G050000involved in cotton resistance to Verticillium wilt
摘要
Abstract
[Objective]This study aims to provide a theoretical basis for molecular breeding of disease-resistant cotton by cloning Gh_D11G050000 gene,and to analyze its function and mechanisms in cotton resistance to Verticillium wilt.[Methods]Bioinformatic method was utilized to analyze the sequence characteristics and phylogenetic relationship of Gh_D11G050000.Quantitative real-time polymerase chain reaction(qRT-PCR)was employed to investigate the expression pattern of this gene,and it's expression level changes after infected by Verticillium dahliae.The function of this gene was validated using virus-induced gene silencing(VIGS)technique.Transcriptome sequencing analysis and the detection of relative expression levels of related genes were conducted to explore the mechanisms of disease resistance.[Results]Gh_D11G050000 exhibited close relationship with Gh_A11G049600 and Gr_11G034620 protein.qRT-PCR analysis revealed that Gh_D11G050000 was highly expressed in cotton roots,and the expression level of Gh_D11G05000 was significantly increased after V.dahliae infection.Gh_D11G050000 silenced cotton plants showed reduced resistance against V.dahliae,characterized by aggravated browning of stem vascular bundles,increased the number of stem segments with bacterial propagation,as well as significantly increased the rate of diseased plants and disease index.Transcriptome analysis combined with qRT-PCR demonstrated that Gh_D11G050000 silenced cotton plants showed decreased transcription levels for several genes in jasmonic acid(JA)signaling pathway,ethylene(ET)signaling pathway,and lignin synthesis pathway.[Conclusion]Gh_D11G050000 positively regulates cotton resistance to Verticillium wilt by influencing the expression of several genes involved in lignin synthesis,JA,and ET signaling pathways.关键词
陆地棉/黄萎病/Gh_D11G050000/抗病基因/病毒诱导的基因沉默/茉莉酸/乙烯/木质素Key words
upland cotton/Verticillium wilt/Gh_D11G050000/resistance gene/virus-induced gene silencing/jasmonic acid/ethylene/lignin引用本文复制引用
黄俊森,马雄风,杨代刚,高俊山,王星星,杨力,裴小雨,贺昆仑,刘艳改,张飞,张先亮,王军..Gh_D11G050000参与棉花黄萎病抗性的功能分析[J].棉花学报,2024,36(3):211-224,14.基金项目
棉花生物育种与综合利用全国重点实验室自主课题:单细胞多组学解析棉花黄萎病防御机制(CB2021E02) （CB2021E02）
新疆维吾尔自治区重点研发任务专项:"双30A"早熟机采棉品种选育与配套技术研究(2022B02052-2) （2022B02052-2）
新疆维吾尔自治区昌吉回族自治州科技支撑产业高质量发展专项:多耐受性高品质棉花新品种选育及配套栽培技术示范推广(2021Z01-01) （2021Z01-01）
国家棉花产业技术体系中早熟品种改良岗位科学家(CARS-15-07) （CARS-15-07）
新疆维吾尔自治区棉花产业技术体系资源研究与育种(早熟棉)岗位专家 （早熟棉）
