甲基磺酸乙酯(EMS)对谷子种子及幼苗活力的影响OA

In order to explore the optimum conditions for EMS mutagenesis of Nenxuan 18 to construct mutant library and accelerate the process of variety breeding,the seeds of Nenxuan 18 were chemically induced by three treatment times and three EMS treatment concentrations,and the mutagenic effects of M1 seed germination and seedling growth were studied.The results showed that compared with the control,the germination rate and seedling growth of Nenxuan 18 were not significantly affected by EMS treatment at a concentration of 0.4%for 6 h.With the increase of EMS soaking concentration and time,seed germination and seedling growth were gradually inhibited.At a concentration of 1.2%,seed soaking for 14 h had the strongest inhibitory effect on seed germination and seedling growth,which greatly reduced the germination potential,germination rate and seedling rate of Nenxuan 18,and led to slow seedling growth.Taking the survival rate of 50%as the standard,the semi-lethal doses of EMS concentration at 6 h,10 h and 14 h soaking time were calculated by regression equation to be 0.99%,0.72%,0.38%,espectively,which could be used as the suitable conditions for the establishment of mutant library by EMS mutagenesis of Nenxuan 18.