南京林业大学学报（自然科学版）2024，Vol.48Issue(4)：37-45,9.DOI:10.12302/j.issn.1000-2006.202406025
铁杉属花粉形态及其聚类分析研究
Phylogenetic relationship and evolutionary patterns of Tsuga pollen morphology:a cluster analysis-based study
摘要
Abstract
[Objective]To analyze the phylogenetic relationships and evolutional patterns of pollen morphology in Tsuga,we observed and compared the pollen characteristics of all 10 extant species of Tsuga along with pollen fossils.[Method]Pollen morphology was examined using scanning electron microscopy(SEM),and hierarchical cluster analysis(HCA)was conducted using seven quantitative indicators and one qualitative indicator.[Result]Tsuga pollens typically exhibits characteristics of the N1 P3 C1 type,with a leptoma on the distal face.The equatorial length ranges from 21.20 to 58.00 μm,predominantly lacking sacci but occasionally with echinae saccate forms.The pollen surface is typically warty or sparsely micro-warty,with spines or without.According to the hierarchical cluster analysis,Tsuga could be divided into three categories:①saccate but lacking echinae type:T.mertensiana and Nothotsuga longibracteata;②lacking sacci and echinae type:T.caroliniana,T.canadensis and pollen fossil T.sp.2;③lacking sacci but echinate type:T.heterophylla,T.ulleungensis,T.diversifolia,T.forrestii,T.sieboldii,T.dumosa,T.chinensis and pollen fossil T.sp.1,T.sp.3.[Conclusion]Pollen morphology in Tsuga has evolved from saccate to non-saccate forms,and from lacking spines to possessing spines.These pollen characteristics are closely related to geographic distribution,indicating similarity among pollen from the same regions.The clustering analysis based on pollen morphology largely aligns with molecular phylogenetic trees,offering a method to distinguish extant species and fossils of Tsuga and providing valuable insights for phylogenetic studies of Tsuga.关键词
铁杉属/花粉形态/扫描电镜/聚类分析Key words
Tsuga/pollen morphology/scanning electron microscopy(SEM)/cluster analysis分类
农业科技引用本文复制引用
陈芷涵,尚鑫,谢淦,张鑫..铁杉属花粉形态及其聚类分析研究[J].南京林业大学学报（自然科学版）,2024,48(4):37-45,9.基金项目
国家植物标本资源库项目(E0117G1001). （E0117G1001）
