同源四倍体菜薹新材料的创制与品质分析OA北大核心CSTPCD

[Objectives]The paper aimed to create a new autotetraploid material with good quality.[Methods]The cotyledon growth point of the diploid Brassica campestris LCX019 seedlings was dripped with 2 g·L-1colchicine solution,the autotetraploid plants were identified by morphological,anatomical,cytological and flow cytometry methods,and the agronomic and quality characteristics of diploid and tetraploid plants were analyzed and compared.[Results]The results of morphology and anatomy showed that the plant type,leaf,flower,seed pod and bolt of the autotetraploid campestris showed"gigantic"characteristics,the stomatal diameter became larger and the stomatal density decreased.The size of pollen grains was larger than diploid,and the shape of pollen grains was rugby ball or irregular.The results of cytology showed that the number of chromosomes in the root tip of tetraploid was about twice that of diploid.Flow cytometry analysis showed that the DNA fluorescence intensity of tetraploid was about twice that of diploid.The contents of soluble sugar,organic acid and cellulose in tetraploid B.campestris significantly increased by 76.17%,13.71%and 80.34%,while the contents of chlorophyll and nitrate nitrogen significantly decreased by 25.01%and 45.10%,respectively,there was no significant change in soluble protein content.By analyzing the light response curve and photosynthetic characteristics of diploid and tetraploid plants,it was found that LCX019 had stronger ability to utilize weak light than diploid plants.[Conclusions]The new tetraploid material with high quality was obtained,which would provide new germplasm resources for the breeding of flowering Chinese cabbage.