南京农业大学学报2024，Vol.47Issue(4)：689-699,11.DOI:10.7685/jnau.202307023
健康与罹病三七内生真菌的分离鉴定及其群落特征差异分析
Isolation,identification and differences in community characteristics of endophytic fungi from healthy and root rot-diseased Panax notoginseng
摘要
Abstract
[Objectives]The aim of this study was to investigate the differences of endophytic fungal community characteristics between healthy and root rot-diseased Panax notoginseng,as well as the distribution pattern in different plant tissues and their changes with growth years.[Methods]1-year-old and 2-year-old of healthy and root rot-diseased P.notoginseng were randomly collected and the endophytic fungi in their roots and stems were isolated by tissue isolation method.Based on morphological characteristics and ITS sequence analysis,the isolated strains were classified and identified,and the community composition and diversity of endophytic fungal community were analyzed.[Results]A total of 966 endophytic fungi strains were isolated and identified,which belonged to 3 phyla,5 classes,14 orders,25 families and 31 genera.The number and isolation rate of endophytic isolates were as follows:diseased plant>healthy plant,2-year-old P.notoginseng>1-year-old P.notoginseng,root>stem.The composition of the culturable endophytic fungi in healthy and root rot-diseased P.notoginseng was quite different,of which the genus Aspergillus,Irpex,Chaetomium,and Penicillium were the dominant endophytic fungi in the healthy P.notoginseng,while the predominant culturable endophytic fungi of root rot-diseased P.notoginseng were Fusarium,Aspergillus,Talaromyces,Chaetomium,and Trichoderma.The composition and diversity of endophytic fungi of P.notoginseng was significantly affected by plant tissues.Specially,the isolation frequency of genus Fusarium from roots of root rot-diseased P.notoginseng was 52%,which was significantly higher than that from the stems of 11.2%.Beneficial fungi such as Trichoderma and Talaromyces were also frequently isolated from diseased roots,with the isolation frequencies of 11.0%and 14.5%,respectively.Moreover,the diversity,richness,and evenness of endophytic fungal community in stems of P.notoginseng were higher than those in roots.With the increase of growth years,the composition and diversity of endophytic fungal community of healthy and root rot-diseased P.notoginseng also changed,and the isolation frequency of Fusarium oxysporum and F.solani in diseased P.notoginseng increased greatly,reaching 14.5%and 32.9%,respectively.Furthermore,the diversity,richness,and evenness of endophytic fungal community in healthy P.notoginseng increased gradually with growth year,while the diversity and evenness of endophytic fungal community in root rot-diseased P.notoginseng were gradually declined.[Conclusions]P.notoginseng harbored a rich diversity of endophytic fungi and its colonization quantity and community composition were significantly distinct between healthy and root rot-diseased plants.Moreover,plant tissues and growth ages also significantly affected the diversity and composition of endophytic fungal community.关键词
三七/内生真菌/根腐病/多样性/生长年限Key words
Panax notoginseng/endophytic fungi/root rot-disease/diversity/growth year分类
农业科技引用本文复制引用
林于蓝,张园可心,王宝英,黄杜鹃,林广宁,魏富刚,张金波,蔡祖聪,赵军..健康与罹病三七内生真菌的分离鉴定及其群落特征差异分析[J].南京农业大学学报,2024,47(4):689-699,11.基金项目
国家自然科学基金项目(42077031,42090065) （42077031,42090065）
云南省专家工作站建设项目(202205AF150039) （202205AF150039）
江苏省卓越博士后计划资助项目(2023ZB047) （2023ZB047）
江苏省研究生科研与实践创新项目(KYCX22_1568,KYCX22_1569) （KYCX22_1568,KYCX22_1569）
