南京农业大学学报2024，Vol.47Issue(4)：700-709,10.DOI:10.7685/jnau.202309013
饲料中添加猪源胆汁酸对肉鸡体内LPS外排功能的影响
Effect of supplementing feed with porcine bile acids on LPS efflux function in broiler chickens
摘要
Abstract
[Objectives]This study aimed to investigate whether bile acids could promote the excretion of lipopolysaccharides(LPS)in broiler chickens and alleviate LPS-induced inflammatory responses.[Methods]Sixty 1-day-old Ross 308 male broiler chickens were divided into four groups,each consisting of 15 chickens:the control group(CON),and the model group(LPS)fed with basal diet,the low-dose bile acid alleviation group(LBA)fed with a basal diet containing 160 mg·kg-1 of porcine bile acid(BA),and the high-dose bile acid alleviation group(HBA)fed with a basal diet containing 250 mg·kg-1 of porcine bile acid.On the 28th day of the experiment,after a 9-hour fasting period,the CON group received intraperitoneal injection of an equivalent volume of physiological saline,while the LPS group,LBA group and HBA group received intraperitoneal injection of LPS(1 mg·kg-1 BW).Three hours after LPS injection,blood was drawn from the wing vein,and all chickens were slaughtered for sampling to detect inflammatory related indicator level,LPS levels,and the expression levels of genes associated with the optake and excretion of LPS in liver.[Results]The liver in the CON group did not exhibit inflammatory cell infiltration.In the LPS group,the liver showed extensive inflammatory cell infiltration,and serum IL-1β levels significantly increased(P<0.05).Additionally,serum and liver LPS levels and liver mannose receptor C1 gene(MRC1),stabilin 1/2 gene(STAB 1/2)expression levels all significantly increased(P<0.05).Moreover,the concentrations of total bile acids(TBA)in serum,liver,bile,and cecal contents significantly decreased(P<0.05).The gene expression levels of liver bile salt export pump(BSEP),multidrug resistance protein 2(MRP2),multidrug resistance-associated protein 1(MDR1),liver X receptor α(LXRα),and farnesoid X receptor(FXR)all significantly decreased(P<0.05).Compared with the LPS group,the LBA group reduced inflammatory cell infiltration in the liver(P<0.05)and significantly decreased in serum IL-1β concentration(P<0.05).Furthermore,serum and liver LPS levels,as well as the gene expression levels of liver MRC1 and STAB1/2,significantly decreased(P<0.05).In the HBA group,inflammatory cell infiltration disappeared in the liver,and both serum IL-1β concentration and the gene expression levels of liver MRC1 and STAB1/2 significantly decreased(P<0.05).Serum LPS level significantly decreased(P<0.05),while bile LPS levels significantly increased(P<0.05).Additionally,the concentrations of total TBA in serum,bile,and cecal contents,as well as the gene expression levels of liver MDR1,ATP-binding cassette transporter G2(ABCG2),and FXR protein expression levels,all significantly increased(P<0.05).[Conclusions]In summary,bile acids enhance liver uptake,synthesis,and excretion functions,improving the efficiency of LPS excretion in broiler chickens and consequently alleviating inflammatory response.关键词
胆汁酸/肉鸡/炎症反应/脂多糖/外排功能Key words
bile acids/broiler chicken/inflammation response/lipopolysaccharide/efflux function分类
农业科技引用本文复制引用
陈良,赵敏蝶,曹爱智,王建民,赵茹茜..饲料中添加猪源胆汁酸对肉鸡体内LPS外排功能的影响[J].南京农业大学学报,2024,47(4):700-709,10.基金项目
国家自然科学基金项目(32272962,31972638) （32272962,31972638）
