南京农业大学学报2024，Vol.47Issue(4)：721-730,10.DOI:10.7685/jnau.202308022
饲粮添加芦丁对肉仔鸡免疫功能和空肠形态、黏膜屏障、抗氧化功能的影响
Effects of dietary rutin supplementation on immune function,jejunum morphology,mucosal barrier function and antioxidant capacity of broilers
摘要
Abstract
[Objectives]Previous studies had found that dietary supplementation of 250-1 000 mg·kg-1 rutin could improve the growth performance of broilers at 21 days of age.This study further investigated the effects of rutin on jejunal morphology,mucosal barrier function and antioxidant capacity of broilers.[Methods]A total of 256 1-day-old male Arbor Acres(AA)broilers with similar body weight(43±1)g were randomly divided into 4 groups with 8 replicates per group and 8 chickens per pen.Broilers in the control group were fed a basal diet,and those in experimental groups were fed the basal diet supplemented with 250,500 and 1 000 mg·kg-1 rutin,respectively.At 21 days of age,one broiler from each replicate was chosen and slaughtered to analyze the indices of intestinal barrier function,immune inflammation,and antioxidant capacity and other related indices.[Results]Compared with the control group,dietary 250 mg·kg-1 rutin significantly increased spleen organ index,total superoxide dismutase(T-SOD)activity and total antioxidant capacity(T-AOC)of jejunal mucosa and closed small cyclic protein-1(ZO-1)in jejunal mucosa of broilers,blocking protein(OCLN),nuclear factor E2-related factor 2(Nrf2)and SOD mRNA expression levels,while serum tumor necrosis faction-α(TNF-α)and D-lactic acid(D-LA)levels,and the expression of TNF-α mRNA in jejunal mucosa significantly decreased(PT<0.05).Compared with the control group,dietary 500 mg·kg-1 rutin significantly increased bursal organ index,jejunum VH/CD value,T-SOD activity and T-AOC in jejunal mucosa,B-cell lymphoma gene-2(Bcl-2),ZO-1 and Nrf2 mRNA expressions in jejunal mucosa of broilers.The serum TNF-α level significantly decreased(PT<0.05).Compared with the control group,250 and 500 mg·kg-1 rutin group,dietary 1 000 mg·kg-1 rutin supplementation significantly increased the serum immunoglobulin M(IgM)and immunoglobulin G(IgG)contents(PT<0.05).Compared with 500 mg·kg-1 rutin group,dietary 250 mg·kg-1 rutin significantly increased spleen organ index,serum lipopolysaccharide(LPS)level and OCLN mRNA expression level,and serum D-LA level of broilers significantly decreased(PT<0.05).Compared with 1 000 mg·kg-1 rutin group,dietary 250 mg·kg-1 rutin significantly increased spleen organ index,and TNF-α mRNA expression level in jejunal mucosa of broilers decreased(PT<0.05).Compared with 1 000 mg·kg-1 rutin group,dietary 500 mg·kg-1 rutin significantly increased the expression of Bcl-2 mRNA level in jejunal mucosa,and the serum LPS level of broilers significantly decreased(PT<0.05).[Conclusions]Dietary supplementation of 250 mg·kg-1 or 500 mg·kg-1 rutin could promote the development of immune organs,enhance immune capacity,improve jejunum morphology,and enhance jejunum barrier function of broilers.The improvement of antioxidant function of jejunum of broilers might be related to the activation of Nrf2 signaling pathway.The optimum dosage of rutin was 250 mg·kg-1 under the experimental conditions.关键词
肉鸡/芦丁/肠道功能/免疫/抗氧化Key words
broiler/rutin/intestinal function/immune/antioxidant分类
农业科技引用本文复制引用
马龙飞,陈顺,庄苏,周斌斌,刘慧娟,张佳琦,王恬,王超..饲粮添加芦丁对肉仔鸡免疫功能和空肠形态、黏膜屏障、抗氧化功能的影响[J].南京农业大学学报,2024,47(4):721-730,10.基金项目
国家自然科学基金项目(31972598) （31972598）
江苏省苏北科技计划项目(SZ-HA2021041) （SZ-HA2021041）
