南京农业大学学报2024，Vol.47Issue(4)：731-740,10.DOI:10.7685/jnau.202305002
慢性热应激对肉鸡盲肠菌群结构和代谢的影响及日粮添加胍基乙酸作用效果研究
Effects of chronic heat stress on the cecal microflora structure and metabolism of broiler and effects of dietary supplementation guanidinoacetic acid
摘要
Abstract
[Objectives]The composition and diversity of gut microbiota have important effects on the development and health of the intestine.This study was conducted to evaluate the effects of chronic heat stress on the cecal microflora structure and metabolism of broiler and the alleviative effect of dietary supplementation with guanidinoacetic acid(GAA).[Methods]A total of 192 28-day-old Arbor Acres(AA)broilers were divided into four treatment groups:normal control(NC,ad libitum feeding and water,22℃),heat stress group(HS,ad libitum feeding and water,32℃),pair-fed group(PF,received food equivalent to that consumed by the HS group on the previous day,and ad libitum water,22℃),guanidinoacetic acid group(HG,ad libitum feeding and water,supplementing the basal diet with 0.6 g·kg-1 GAA,32℃).Each treatment consisted of 6 replicates with 8 broilers per cage.The trial lasted for 14 days.[Results]At the phylum level,the relative abundance of Firmicutes and Actinobacteria in the HS group was significantly higher than those in the NC and PF groups,while the relative abundance of Bacteroidota was significantly lower(P<0.05).LEfSe analysis revealed that Oscillospiraceae and Erysipelatoclostridium,which were associated with inflammation,were enriched in the HS group.Compared with the NC group,thermal exposure significantly increased the concentration of acetic acid and significantly decreased the concentration of butyric acid(P<0.05).The relative abundance of Actinobacteria decreased,and the concentrations of acetic acid decreased while butyric acid concentrations increased in the HG group compared with HS group(P<0.05).Geobacillus and Bacillaceae,which were associated with anti-inflammatory effects,were enriched in the HG group.[Conclusions]Chronic heat stress changed cecal microflora structure and short-chain fatty acid metabolism.Dietary supplementation with GAA was effective in alleviating heat stress which induced the caecal structural and short-chain fatty acid metabolic changes.关键词
肉鸡/胍基乙酸/慢性热应激/盲肠微生物Key words
broiler/guanidinoacetic acid/chronic heat stress/cecal microflora分类
畜牧业引用本文复制引用
彭旭艺,邢通,李蛟龙,张林,赵良,江芸,高峰..慢性热应激对肉鸡盲肠菌群结构和代谢的影响及日粮添加胍基乙酸作用效果研究[J].南京农业大学学报,2024,47(4):731-740,10.基金项目
国家自然科学基金项目(32072780) （32072780）
江苏现代农业产业技术体系建设专项资金(JATS[2023]418) （JATS[2023]418）
