农业生物技术学报2024，Vol.32Issue(8)：1742-1752,11.DOI:10.3969/j.issn.1674-7968.2024.08.003
谷子KNOX基因家族鉴定及组织表达模式分析
Identification and Tissue Expression Pattern Analysis of KNOX Gene Family in Setaria italica
摘要
Abstract
Knotted1-like homeobox(KNOX)is a transcription factor unique to plants,characterized by its homologous domain,and is widely involved in plant growth and development.To explore the potential function of the foxtail millet(Setaria italica)KONXs,bioinformatic methods were used to identify and analyze the SiKNOX gene family at the whole-genome level of foxtail millet,and the expression patterns of SiKNOXs in different tissues were also detected using RT-qPCR.The results showed that a total of 11 SiKNOXs were identified in the S.italica genome.Phylogenetic analysis classified them into 2 groups,classⅠand classⅡ.ClassⅠcontained 5 SiKNOXs and classⅡcontained 6 SiKNOXs.Subcellular localization prediction revealed that all SiKNOX proteins were predicted to be nuclear.Chromosomal localization analysis showed that they were unevenly distributed among the 7 chromosomes of S.italica.Duplication analysis showed that there were 5 SiKNOXs forming 4 segmental duplication gene pairs.Gene structure analysis revealed that all 11 SiKNOXs consisted of 5～6 exons.Protein conserved domain analysis indicated 10 motifs in SiKNOXs.Motifs 2,3,5,and 6 corresponded to the KNOX1,KNOX2,ELK,and HD domains,respectively.The promoter region of the SiKNOXs harbored numerous cis-acting elements that were closely associated with various biological processes.Expression pattern analysis revealed that all SiKNOXs were detected in different tissues(including roots,stems,leaves,tassels,and seeds),and most of them showed tissue-specific expression.This study lays the foundation for further research on KNOX genes family in the growth and development of S.italica.关键词
谷子/KNOX(knotted1-like homeobox)/基因家族/组织表达模式Key words
Setaria italica/Knotted1-like homeobox(KNOX)/Gene family/Tissue expression pattern分类
农业科技引用本文复制引用
施卫萍,张欣,赵媛,刘敏,代渴丽,张艾英,郭二虎,史关燕,郭杰..谷子KNOX基因家族鉴定及组织表达模式分析[J].农业生物技术学报,2024,32(8):1742-1752,11.基金项目
国家自然科学基金(32201857) （32201857）
山西省重点研发计划(2022ZDYF107) （2022ZDYF107）
山西省基础研究计划(20210302124019) （20210302124019）
山西省现代农业产业技术体系建设专项(2023CYJSTX04) （2023CYJSTX04）
山西省高等学校科技创新(2021L120) （2021L120）
山西农业大学科技创新基金(2020BQ60) （2020BQ60）
