农业生物技术学报2024，Vol.32Issue(8)：1878-1889,12.DOI:10.3969/j.issn.1674-7968.2024.08.015
异育银鲫'中科5号'产消化酶益生菌的筛选、鉴定及生物学特性研究
Screening,Identification and Biological Characteristics Research of Digestive Enzyme Secreting Probiotics from Gibel Carp(Carassius auratus gibelio)CASⅤ
摘要
Abstract
The low feed digestibility and absorption rate is an important reason for high feed cost and low profit of fish farming.Gibel carp(Carassius auratus gibelio)is one of the main current crucian carp breeding in China,the aim of this study was to screen safe and efficient probiotics which can secret digestive enzymes from healthy culture system of gibel carp CASⅤ,so as to improve feed digestion and absorption capacity of CASⅤand reduce feed cost.Ten strains with protease,lipase and amylase activities were isolated from CASⅤ and pond water by spot inoculation method.Two highly efficient,non-hemolytic,sensitive to most antibiotics and non-pathogenic strains,ZKW7 and ZKF17,were obtained by further analysis of enzyme secretion ability and safety test(hemolysis,antibiotics susceptibility and intraperitoneal infection).Based on 16S rDNA sequence analysis,both strains had the highest similarity to Bacillus subtilis and B.halotolerans.The results of biological characteristics analysis showed that both strains had strong degrading activities for NH4+,NO2-and NO3-.The optimum growth conditions of ZKW7 were pH 8,salinity 15 and Fe2+in culture medium,respectively.By contrast,the optimum growth conditions of ZKF17 were pH 7,salinity 10～30 and the presence of Mg2+.In addition,these 2 strains were resistant to acid,bile salt,intestinal fluid and succus gastricus to some extent.In the present study,2 strains of safe and efficient enzyme-producing Bacillus sp.were obtained,which could be used as potential probiotics and would be conducive to the healthy farming of C. auratus gibelio var. CASⅤ.关键词
异育银鲫/产酶菌/安全性/生物学特性Key words
Carassius auratus gibelio/Enzyme secreting bacteria/Safety/Biological characteristics分类
水产学引用本文复制引用
于鹏,马晶晶,成钰,张成硕,王凯,周正海..异育银鲫'中科5号'产消化酶益生菌的筛选、鉴定及生物学特性研究[J].农业生物技术学报,2024,32(8):1878-1889,12.基金项目
中国盐城渔业高质量发展研究课题(YCSCYJ2021029) （YCSCYJ2021029）
