曲阜师范大学学报（自然科学版）2024，Vol.50Issue(3)：112-116,5.DOI:10.3969/j.issn.1001-5337.2024.3.112
水生植物红宫廷草的快速繁殖研究
A study on rapid propagation of aquatic plant Rotala rotundifolia'Gontin'by tissue culture
摘要
Abstract
Rotala rotundifolia'Gontin'is a kind of aquatic plants,which has high ornamental and eco-logical value,but the traditional propagation method is difficult to meet the market demand.In order to ob-tain a large number of R.rotundifolia'Gontin'seedlings in a short period of time,the buds were selected as explants,the optimum propagation method of plant tissue culture was studied using MS(Murashige and Skoog)as basic medium.The results showed that MS with 0.5 mg/L 6-BA(6-Benzylaminopurine)was the optimal medium for bud proliferation,with 21.1 buds and 9.83 mg/L chlorophyll content.Adding 1 g/L Activated carbon(AC)in MS medium was beneficial to rooting,and the optimum medium for rooting was MS with 1 g/L AC and 0.6 mg/L IBA(Indole-butyric acid).The transplant survival rate was 100%.In this research,a high frequency establishment of in vitro propagation system on R.rotundifolia'Gontin'will provide a reliable technical support for its large scale production and utilization.关键词
红宫廷草/组织培养/增殖/生根Key words
Rotala rotundifolia'Gontin'/tissue culture/proliferation/rooting分类
农业科技引用本文复制引用
杨水兰,杨茗越,徐月瑶,于琨,韦浩,刘柏玲..水生植物红宫廷草的快速繁殖研究[J].曲阜师范大学学报（自然科学版）,2024,50(3):112-116,5.基金项目
国家自然科学基金(31700624) （31700624）
林木遗传育种国家重点实验室(中国林业科学研究院)开放课题(TGB2021005). （中国林业科学研究院）
