基于RNA-Seq的甘蔗响应鞭黑粉菌侵染的早花相关基因挖掘OA北大核心CSTPCD

Sporisorium scitamineum,the pathogen of destructive disease smut,usually causes abnormal growth of mer-istematic tissue and forms a"black whip"in meristem tissues.In some cases,it induces formation of flowering struc-tures in some sugarcane cultivars(genotypes),but the exact mechanism remains unclear.In order to explore flowering related genes and the expression patterns in sugarcane in response to infection by S.scitamineum,the healthy and S.scitamineum infected'guitang42'sugarcane cultivar samples were analyzed by transcriptome sequencing.A total of 3276 differentially expressed genes(DEGs)were identified,of which 1677 genes were up-regulated(P＜0.05)and 1599 genes down-regulated(P＜0.05).The DEGs were mostly enriched in the biosynthesis process,ribosome,ribonucleopro-tein complex,ribosome structural components,etc.according to GO enrichment analysis.The KEGG analysis showed that the DEGs were mostly enriched in amino acid biosynthesis,purine metabolism,carbon metabolism,phagocytosis,and other metabolic pathways.Also,out of 30 DEGs were identified to be associated with flowering,and the represen-tative genes such as FT1,PIE1,GID1,GA20ox-1,GA20ox-2 were validated by qRT-PCR.The qRT-PCR results were consistent with the transcriptomic data,confirming the dependability of the transcriptome sequencing results.The find-ings would present essential candidate genes for revealing the mechanism of early flowering of sugarcane plants caused by S.scitamineum,and lay the foundation for disease resistance breeding.