热带作物学报2024，Vol.45Issue(6)：1273-1281,9.DOI:10.3969/j.issn.1000-2561.2024.06.019
大粒咖啡炭疽病病原菌形态和分子鉴定及杀菌剂筛选
Morphological and Molecular Identification of Colletotrichum kaha-wae Causing Anthracnose of Coffea liberica and Fungicide Selection for the Disease Management
摘要
Abstract
The present work was conducted to identify the pathogen causing anthracnose of Liberica coffee(Coffea liberica)plants and to screen fungicides against the disease pathogen.The leaves of C.liberica with anthracnose symp-toms were sampled from fields for fungal isolation and purification.Four pure fungal isolates were obtained and only KFTJ01 was verified pathogenic to the coffee leaves by tests following Koch's Postulates.The rDNA-ITS sequence(554 bp),beta-tubulin gene(tub2)and calmodulin gene(CAL)were successfully amplified via PCR from genomic DNA of KFTJ01 and sequenced.Blastn analysis of the the three DNA sequences showed that KFTJ01 was identical to the strain WZ-135(99.62％,MN856281),CREADC-ER2212(100％,MT409131)and YMTJ4(100％,MK569149)of Colle-totrichum kahawae.KFTJ01 was also clustered with C.kahawae WZ-135 strain on the same end-branch of the phylogenic tree(bootstrap confidence is 98％).Therefore,the pathogen of Liberica coffee was identified as C.kahawae which is a quarantine species and has never been recorded in China.Fungicide selection experiments showed that among the 10 chemicals,methionine-acetazolyl and methyl thiobacillam almost completely(100％)suppressed the colony growth of KFTJ01.The median effective concentration calculated from the functions was 0.0607 mg/mL and 0.0809 mg/mL,respec-tively,indicating that both the chemicals were very effective against the anthracnose pathogen.The results are important references for the field diagnosis of the coffee plant anthracnose and for controlling the disease epidemics.关键词
大粒咖啡/炭疽病/卡哈瓦刺盘孢/多基因序列分析/杀菌剂Key words
Coffea liberica/anthracnose/Colletotrichum kahawae/multigene sequence analysis/fungicide分类
农业科技引用本文复制引用
李学俊,任圆,陈红梅,曲鹏,杜华波,何鹏搏,谭万忠..大粒咖啡炭疽病病原菌形态和分子鉴定及杀菌剂筛选[J].热带作物学报,2024,45(6):1273-1281,9.基金项目
云南省科技厅重点研发计划项目——云南省咖啡产业绿色发展国际联合研发中心(No.202303AP140010) （No.202303AP140010）
云南省教育厅基础研究项目(No.2023Y0911). （No.2023Y0911）
