热带作物学报2024，Vol.45Issue(7)：1303-1313,11.DOI:10.3969/j.issn.1000-2561.2024.07.001
木薯MeAHL17基因的克隆及表达分析
Cloning and Expression Analysis of MeAHL17 Gene in Cassava
摘要
Abstract
AT-hook nuclear localized proteins(AHLs)are small DNA-binding protein motifs that play an important role in plant growth and development,organ construction,stress and hormone signaling response.In this study,MeAHL17 was cloned from cassava cultivar SC8.Bioinformatics methods were used to analyze MeAHL17 promoter,protein phys-icochemical properties,prediction of conserved functional domain and sequence comparison.The results showed that the open reading frame length of MeAHL17 gene was 939 bp,encoding a protein with 312 amino acids,its theoretical isoelectric point was 6.89,its molecular formula was C1420H2215N415O451S11,and its molecular weight was 32.669 38 kDa.The total number of positively charged amino acid residues(Lys+Arg)was 23,the total number of negatively charged amino acid residues(Asp+Glu)was 24,the fat coefficient was 57.47,the total average hydrophilic coefficient(GRAVY)was-0.491 and the instability coefficient was 58.06.The protein contains no signal peptide,is located in the cell mem-brane,has no transmembrane structure,contains 5 glycosylation sites and 45 phosphorylation sites,and is an unstable hydrophilic acidic protein MeAHL17 contained AT-hook conserved core sequences and PPC domain conserved core sequences,which was consistent with the typical structural characteristics of AT-hook family.Through subcellular lo-calization and transcriptional activity analysis experiments,MeAHL17 was proved to be a nucleus localized transcrip-tion factor with transcriptional activity.Tissue expression pattern analysis showed that MeAHL17 was mainly expressed in somatic embryo,fibrous root and callus.Different hormone treatments showed that MeAHL17 was induced by ethyl-ene(ACC),methyl jasmonate(JA)and growth hormone(IAA),which suggested that MeAHL17 might be involved in ethylene,methyl jasmonate and growth hormone signaling pathway.Abiotic stress treatment showed that MeAHL17 was responsive to drought and salt stress.This study preliminarily identified the important role of MeAHL17 in growth and development,hormone signaling and stress,and provided theoretical basis and reference for further study of its function.关键词
木薯/MeAHL17/基因克隆/亚细胞定位/转录活性分析/表达模式分析Key words
cassava/MeAHL17/gene cloning/subcellular localization/transcriptional activity analysis/expression pat-tern analysis分类
农业科学引用本文复制引用
张亚文,王晓彤,张兴龙,唐湘宁,刘姣,郭建春..木薯MeAHL17基因的克隆及表达分析[J].热带作物学报,2024,45(7):1303-1313,11.基金项目
国家自然科学基金项目(No.32001602) （No.32001602）
海南省自然科学基金项目(No.323MS087) （No.323MS087）
现代农业产业技术体系建设专项(No.CARS-11-HNGJC). （No.CARS-11-HNGJC）
