钻喙兰属种质资源鉴评与分子身份证构建OA北大核心CSTPCD

Rhynchostylis is an epiphytic orchid belonging to the Orchidaceae family.It possesses high ornamental and economic value.In this study,based on the collection of 10 different germplasm resources from three common species of Rhynchostylis,21 pairs of SSR polymorphic primers were selected using fluorescent-labeled capillary electrophoresis for allele variation detection.Among them,13 primer pairs exhibited high polymorphism,resulting in a total of 47 am-plified alleles.The average number of observed alleles(Na)was 2.238,and the average number of effective alleles(Ne)was 1.860.The observed heterozygosity(Ho)of the 21 loci ranged from 0 to 0.778,with a mean of 0.421,while the expected heterozygosity(He)ranged from 0.148 to 0.574,with a mean of only 0.340.The Shannon index(Ⅰ)ranged from 0.096 to 0.987,with a mean of 0.567.The clustering results and PCoA analyses of the 10 germplasm were consis-tent with the traditional botanical classification,and all the germplasm could be classified into three taxa by species.On this basis,four pairs of core primers were selected to construct a molecular ID for each germplasm.This study lays a foundation for the collection,conservation,identification,development and utilization of the germplasm resources of Rhynchostylis.