日用化学工业（中英文）2024，Vol.54Issue(7)：873-878,6.DOI:10.3969/j.issn.2097-2806.2024.07.016
UPLC-MS/MS法测定化妆品中5种美白剂
Determination of 5 whitening agents in cosmetics using UPLC-MS/MS
摘要
Abstract
A method was established for quantitative determination of five whitening agents in cosmetics using ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The samples were extracted with methanol-water solution(70∶30),ultrasonic extraction for 30 minutes and then separated by Agilent column(ZORBAX RR StableBond Aq)followed by gradient elution.An electrospray ionization(ESI)source in negative ion mode was utilized for multiple reaction monitoring(MRM)scanning.The results show that under optimal conditions no obvious matrix effect exists in 3 different cosmetic matrices.Among the three cosmetics with different types of matrices,good linearity is obtained for five whitening agents in the range of 1.0-200.0 ng/mL,and the correlation coefficients(R2)are more than 0.996.The limit of detection(LOD)and limit of quantitation(LOQ)are 0.01-0.1 mg/kg and 0.03-0.3 mg/kg,respectively.Spike recovery is performed on three cosmetics at three different spike levels(1,2,and 10 times of the LOQ),and the average recovery range is 82.6%-104.8%with a relative standard deviation of 2.1%-8.6%(n=6).This method is easy to implement,efficient and accurate.It is suitable for quantitative analysis of five whitening agents,including deoxyarbutin,α-arbutin,β-arbutin,hydroquinone,and ursolic acid in common cosmetics,providing technical support for the regulation and risk monitoring of the cosmetics industry.关键词
超高效液相色谱-四极杆串联质谱法/美白剂/化妆品Key words
UPLC-MS/MS/whitening agents/cosmetics分类
化学化工引用本文复制引用
王昌钊,李子豪,王一欣,杨玥..UPLC-MS/MS法测定化妆品中5种美白剂[J].日用化学工业（中英文）,2024,54(7):873-878,6.基金项目
陕西省重点研发计划项目资助(2022GY-321) （2022GY-321）
