日用化学品科学2024，Vol.47Issue(7)：38-42,76,6.DOI:10.3969/j.issn.1006-7264.2024.07.009
高山火绒草类囊泡的提取、表征及其生物活性的检测
Extraction and characterization of the extracellular vesicles derived from Leontopodium Alpinum(Edelweiss)callus culture supernatant and evaluation of their biological efficacy in Vitro
赵旺宇 1张旭辉 1张振兴 1高琦1
作者信息
- 1. 北京尧景基因技术有限公司,北京 102699
- 折叠
摘要
Abstract
The study aims to study the extraction,characterization and biological activity of vesicles from Leontopodium alpinum.The sacs of Leontopodium Alpinum were extracted from the supernatant of Leontopodium Alpinum callus culture using tangential flow clarification,concentration process,and column chromatography.The cytotoxicity,antioxidant capacity,promotion of Col1a1 protein production,inhibition of matrix metalloproteinase-1(MMP-1)expression,and promotion of cell migration ability of Leontopodium Alpinum vesicles were tested.The results showed that high-purity thylakoids could be obtained from the supernatant of the callus culture of Leontopodium Alpinum.The thylakoids of Leontopodium Alpinum had significant antioxidant,Col1a1 protein production,and matrix metalloproteinase-1(MMP-1)expression inhibition,cell migration promotion,and other biological activities at the concentration of 500 μg/mL.关键词
高山火绒草/类囊泡/切向流澄清/基质金属蛋白酶-1/抗氧化Key words
edelweiss/vesicles/tangential flow clarification/MMP-1/anti-oxidation分类
化学化工引用本文复制引用
赵旺宇,张旭辉,张振兴,高琦..高山火绒草类囊泡的提取、表征及其生物活性的检测[J].日用化学品科学,2024,47(7):38-42,76,6.
