西华大学学报（自然科学版）2024，Vol.43Issue(4)：110-122,13.DOI:10.12198/j.issn.1673-159X.5515
巴戟天提取物对地塞米松诱导小鼠肌少症的治疗效果及作用机制研究
The Therapeutic and Mechanistic Study of the Extract of Morinda Officinalis on Dexamethasone-Induced Sarcopenia in Mice
摘要
Abstract
Objective:This study aims to explore the therapeutic effect of Morinda officinalis extract(MoE)on dexamethasone(Dex)-induced sarcopenia in mice and its possible mechanism of action.Meth-ods:Firstly,the pharmacological components and important targets of Morinda officinalis were investig-ated using network pharmacology to predict the mechanism of action of MoE.In the animal experiment,50 KM mice were randomly divided into 5 groups(control,sarcopenia,MoE-H,MoE-M,MoE-L).Except for the control group,the other groups were subcutaneously injected with Dex for 45 days to induce sarcopenia,and the three treatment groups were simultaneously orally administered different doses of MoE.During the experiment,the animals'body weight,exercise ability(treadmill and grip strength),and body composition were regularly measured.After euthanasia,the muscle coefficient of the hind limbs was calculated,and his-tological and related gene expression tests were performed.Results:The results of network pharmacology suggested that MoE may treat sarcopenia by upregulating the IGF-1/AKT/mTOR signaling pathway.The animal experiment results show that MoE significantly reverses the decrease in body weight and muscle coefficient caused by Dex,improved motor ability,improved histological lesions,upregulated the expres-sion of genes such as IRS-1,mTOR,MFN2,OPA1,MYOD,and MYOG,and downregulated the expres-sion of genes such as Atrogin-1,MuRF-1,and MSTN.Conclusion:MoE has a therapeutic effect on Dex-induced muscle atrophy,improving histological morphology,and its mechanism of action may involve the activation of the IGF-1/AKT/mTOR pathway.关键词
巴戟天提取物/地塞米松/肌少症/网络药理学/IGF-1/AKT/mTOR通路Key words
Morinda officinalis/dexamethasone/sarcopenia/network pharmacology/IGF-1/AKT/mTOR pathway分类
医药卫生引用本文复制引用
赖朋,方雅静,李昕怡,叶明珠,刘丹..巴戟天提取物对地塞米松诱导小鼠肌少症的治疗效果及作用机制研究[J].西华大学学报（自然科学版）,2024,43(4):110-122,13.基金项目
教育部春晖计划项目(14205506). （14205506）
