西华大学学报（自然科学版）2024，Vol.43Issue(4)：123-136,14.DOI:10.12198/j.issn.1673-159X.5505
HPLC-MS/MS法测定茶叶中花青素类活性物质含量
Determination of Anthocyanin-type Active Substances Contents in Tea by HPLC-MS/MS Method
摘要
Abstract
Anthocyanin,as one of the major bioactive components in tea,represents the edible value.Due to the enrichment of tea in protein,sugar,tea polyphenols,flavonoids,alkaloids,minerals,vitamins,pigments and other components,it is difficult to determine the anthocyanin active substances in tea matrix by relevant domestic standards.Thus,this paper established a high-performance liquid chromatography-tan-dem mass spectrometry(HPLC-MS/MS)method for the detection of six anthocyanin compounds in tea sample under the optimization of pretreatment conditions and instrument conditions.The established meth-od showed a good linear relationship within the concentration range of 25 to 2500 μg/L,with correlation coefficients(R)greater than 0.99,recovery rates ranging from 91.95%to 106.99%,and precision RSD less than 10%,which meet the requirements for analytical performance.The method exhibited advantages such as easy operation,high sensitivity,and high accuracy.These advantages make it suitable for related analyt-ical detections and providing valuable guidance for the establishment of standard methods for anthocyanin content detection in tea.关键词
花青素类/茶叶/活性物质/高效液相色谱-串联质谱Key words
anthocyanins/tea/active substances contents/HPLC-MS/MS分类
化学化工引用本文复制引用
黄玉坤,汪国胜,骆丝雨,吉海阿各,胡超琼,冯德建,邹燕,叶善蓉..HPLC-MS/MS法测定茶叶中花青素类活性物质含量[J].西华大学学报（自然科学版）,2024,43(4):123-136,14.基金项目
四川省重点研发项目(2020YFN0154) （2020YFN0154）
茶叶标准与检测技术四川省重点实验室开放课题(STTT202103). （STTT202103）
