水产科技情报2024，Vol.51Issue(4)：205-210,6.DOI:10.16446/j.fsti.20240300129
基于PCR技术检测复合四倍体鲫及其亲本的分子标记开发
Development of molecular markers for detecting multiple tetraploid allogynogenetic crucian carp and its parentage based on PCR technology
摘要
Abstract
In order to establish a simple and rapid method to distinguish between multiple tetraploid allogynogenetic crucian carp and its parentage,DNA molecular markers were developed and a pair of primers was screened based on the difference of growth-related genes between Carassius auratus and Cyprinus carpio sequences.The DNA fragments of 1 399 bp and 1 125 bp were amplified in Xingguo red carp(C.carpio var.singuonensis)and C.auratus gibelio"CASⅢ",respectively.Moreover,both 1 399 bp and 1 125 bp were amplified in multiple tetraploid allogynogenetic crucian carp.In summary,this molecular marker provides a relatively fast and efficient method for distinguishing between C.carpio var.singuonensis,C.auratus gibelio"CASⅢ"and multiple tetraploid allogynogenetic crucian carp,and also provides assistance for the identification of fry or adult fish in future breeding research.关键词
复合四倍体/分子标记/兴国红鲤/天然三倍体/异精雌核发育Key words
multiple tetraploid/molecular marker/Cyprinus carpio var.singuonensis/natural triploid/allogyno-genesis引用本文复制引用
顾楠,李永强,刘峰..基于PCR技术检测复合四倍体鲫及其亲本的分子标记开发[J].水产科技情报,2024,51(4):205-210,6.基金项目
上海市科技兴农重点攻关项目(2018-02-08-00-07-F01547). （2018-02-08-00-07-F01547）
