四川医学2024，Vol.45Issue(7)：721-725,5.DOI:10.16252/j.cnki.issn1004-0501-2024.07.005
过量甲状腺激素T3对β2-AR基因敲除小鼠骨代谢的影响
Effects of Excess Thyroid Hormone T3 on Bone Metabolism in β2-AR Knockout Mice
摘要
Abstract
Objective To investigate the effect of excess thyroid hormone T3 on bone metabolism in β2-AR knockout(β2-AR-/-)mice.Methods 18 normal C57BL/6J mice were divided into wild type group,wild type+10 times T3 group(35 μg/kg)and wild type+20 times T3 group(70 μg/kg),with 6 mice in each group.Another 18 β2-AR-/-mice were divided into homo-zygote group,homozygote+10 times T3 group(35 μg/kg)and homozygote+20 times T3 group(70 μg/kg),with 6 mice in each group.After 12 weeks of intraperitoneal injection,the wild-type group and the homozygous group were injected with the same a-mount of normal saline.Serum triiodothyronine(TT3),tetraiodothyronine(TT4),alkaline phosphatase(ALP),osteopontin(BGP),bone resorption marker(CTX),tartrate acid phosphatase 5b(TRAP-5b),superoxide dismutase(SOD)and malondialdehyde(MDA)were detected by immunoenzyme linked immunosorbent assay(Elisa).The distal femurs of mice were analyzed by micro-computed tomography(micro-CT),and trabecular bone was calculated,including trabecular pattern factor(Tb.Pf),trabecular number(Tb.N),trabecular separation(Tb.sp),bone thickness(Tb.Th),bone connection density(Conn.Dn)and bone mineral density(BMD).Results Sp was increased,Tb.N,Conn.Dn and BMD were decreased in wild and homozygote mice.Th,Tb.Sp in-creased,Tb.N and Conn.Dn decreased in the tissues of homozygous+20 times T3 mice.Compared with the wild type+10 times T3 group,the levels of TT3,TT4,CTX,TRAP-5b and MDA in the serum of 20 times T3 wild type mice were significantly in-creased,the levels of SOD,BGP and ALP were significantly decreased,the levels of Tb.Pf in the tissues were increased,and Tb.Th in tissues of homozygote+20 times T3 mice were significantly higher than those of homozygote+10 times T3 group(P＜0.05);Th,Tb.N and BMD in tissues of homozygote+20 times T3 group were significantly higher than those of wild type+20 times T3 group,while the levels of TT3,TT4,BGP,SOD and ALP in serum of homozygote+20 times T3 group were significantly higher than those of wild type+20 times T3 group,and the levels of CTX,TRAP-5b and MDA in serum were significantly lower than those of wild type+20 times T3 group while Tb.Pf and Tb.Sp decreased(P＜0.05).Conclusion β2-AR deficiency may prevent abnor-mal bone metabolism caused by excessive T3,reduce oxidative stress injury in vivo,and protect bone morphology.关键词
甲状腺激素/β2-AR/基因敲除/骨代谢Key words
thyroid hormone/β2-AR/gene knockout/bone metabolism分类
医药卫生引用本文复制引用
袁宁,王晓娟,乔彦,何明海,王凤,吉蓉..过量甲状腺激素T3对β2-AR基因敲除小鼠骨代谢的影响[J].四川医学,2024,45(7):721-725,5.基金项目
南充市市校科技战略合作课题(编号:19SXHZ0173) （编号:19SXHZ0173）
