三维荧光结合区域积分鉴别酸枣仁及其掺伪品OACSTPCD

Objective:To establish the identification method of three-dimensional fluorescence spectrum of Suanzaoren(Ziziphi Spinosae Semen),and to provide the basis for the authenticity identification of Suanzaoren.Methods:Three-dimensional fluo-rescence spectra of six batches of Suanzaoren from different areas were established,and two batches of counterfeit Suanzaoren and three batches of adulterated Suanzaoren were identified and analyzed by three-dimensional fluorescence technique.The three-dimensional fluorescence spectra of Suanzaoren from different producing areas,counterfeit and adulterated products of Suanzaoren were integrated by fluorescence region integration method.The regional integral values were analyzed by similarity evaluation,clustering and principal component analysis.Results:The similarity results showed that the similarity of three-dimensional fluorescence spectra of Suanzaoren from different producing areas was 1,and the similarity was very high.The clustering results were consistent with the results of principal component analysis,and the samples were clearly divided into three categories,which could distinguish between genuine,counterfeit and adulterated Suanzaoren by regional integral value.Conclusion:The study on the quality consistency of Suanzaoren from different areas can be realized by three-dimensional fluorescence technology,and the rapid authenticity identification of Suanzaoren can be realized,so as to provide reference for the quality evaluation of Suanzaoren.