二参真武汤对心肾阳虚型慢性心力衰竭大鼠心肌能量代谢的影响OACSTPCD

Objective:Inquire into impact of Ershen Zhenwu decoction on cardiac muscle energy metabolism in ratswith chronic heart failure(CHF)due to Xinshen Yangxu.Methods:Eight rats from 60 rats were stochastically selected as blank group,and the remaining 52 rats were rendered 0.02％Doxorubicin(DOX)intraperitoneal injection after thyroidectomy to establish CHF model of Xinshen Yangxu.The model rats were stochastically split into model group,positive control group,Ershen Zhenwu low-dose group and Ershen Zhenwu high-dose group,with 8 rats in each group.All groups were given corresponding drugs,and course of treatment was 28 days.End of drug administra-tion,serum triiodothyronine(T3),thyroxine(T4),N-terminal B-type natriuretic peptide(NT-proBNP)in blank group and model group.The myocardial Adenine nucleoside triphosphate(ATP),adenosine diphosphate(ADP),B-type natriuretic peptide(BNP),creatine kinase isoenzyme(CK-MB),Na+-K+-ATPase,Ca2+-ATPase each group were measured by ELISA.Cardiac function were detected by color doppler echocardiography.Pathological changes of heart were observed by HE staining.The morphology of myocardial collagen fiber deposition were observed by Sirius staining.Ultrastructure of myocardial mitochondriawas observed by electronmicroscope.Results:After modeling,ser-um NT-proBNP increased and T3 and T4 decreased(P＜0.01).Compared with the blank group,left ventricular ejec-tion fraction(LVEF)and left ventricular short axis shortening rate(LVFS)decreased in the model group,while left ventricular end-systolic inner diameter(LVIDs)and left ventricular end-systolic volume(LVESV)increased(all P＜0.01).ATP,ADP,Na+-K+-ATPase and Ca2+-ATPase decreased,while BNP and CK-MB increased(all P＜0.01).The myocardial cells were swollen,the edge was vague,increase of collagen fiber deposition,mitochondria de-creased and the crista was vague.Compared with the model group,LVEF and LVFS in the high and the low dose-groups of Ershen Zhenwu decoction and the positive control group increased,while LVIDs and LVESV decreased,while ATP,ADP,Na+-K+-ATPase and Ca2+-ATPase increased,BNP and CK-MB decreased(all P＜0.01).Swell-ing myocardial cellsdecreased,collagen fibersdeposition surface area decreased,and myocardial mitochondrial harm was alleviated.Conclusion:Ershen Zhenwu decoction can validly amend myocardial injury and protect heart in CHF rats,and its mechanism may be related to regulate and control ATP and ADP energy metabolism.