化瘀降浊方治疗痰湿型多囊卵巢综合征机制研究OACSTPCD

Objective:Exploring therapeutic effect and mechanism of Huayu Jiangzhuo formula on rats with phlegm dampness type polycystic ovary syndrome(PCOS).Methods:Rat model of phlegm dampness type PCOS con-structed using high-fat diet and letrozole gavage.Fifty rats were randomly divided into control group,model group,metformin group,low-dose Huayu Jiangzhuo formula group,high-dose Huayu Jiangzhuo formula group.Using ELISA method to detect hormone levels in rat serum.HE staining was used to detect pathological changes in rat ovarian tissue.Western blot to detect changes in TLR4/NF-κB p56 signaling pathway protein expression levels.Results:After intervention,compared with the model group,body weight and Lee's index of rats in the metformin group,low-dose Huayu Jiangzhuo formula group,high-dose Huayu Jiangzhuo formula group were significantly lower(P＜0.05).Compared with the control group,the levels of testosterone(T),luteinizing hormone(LH),and LH/FSH in the model group rats were significantly higher,while the levels of estradiol(E2),progesterone(P),and follicle stimulating hormone(FSH)were significantly lower(P＜0.05).Compared with the model group,after interven-tion,the T,LH,LH/FSH levels of rats in the metformin group,low-dose Huayu Jiangzhuo formula group and high-dose Huayu Jiangzhuo formula group decreased(P＜0.05),while the E2,P,and FSH levels increased(P＜0.05).Compared with the control group,the relative expression levels of TLR4,MyD88,and NF-κB p65 proteins in the o-varian tissue of the model group rats increased(P＜0.05).Compared with the model group,the relative expression levels of TLR4,MyD88,and NF-κB p65 proteins in the ovarian tissue of rats in the metformin group,low-dose Huayu Jiangzhuo formula group and high-dose Huayu Jiangzhuo formula group were reduced(P＜0.05).Conclusion:Huayu Jiangzhuo formula can regulate secretion level of sex hormones in PCOS rats by inhibiting activity of TLR4/NF-κB p56 signaling pathway and improve ovarian tissue pathological damage.