陕西中医2024，Vol.45Issue(7)：888-892,5.DOI:10.3969/j.issn.1000-7369.2024.07.005
化瘀降浊方治疗痰湿型多囊卵巢综合征机制研究
Mechanism study on treatment of Tanshi type polycystic ovarian syndrome with Huayu Jiangzhuo formula
摘要
Abstract
Objective:Exploring therapeutic effect and mechanism of Huayu Jiangzhuo formula on rats with phlegm dampness type polycystic ovary syndrome(PCOS).Methods:Rat model of phlegm dampness type PCOS con-structed using high-fat diet and letrozole gavage.Fifty rats were randomly divided into control group,model group,metformin group,low-dose Huayu Jiangzhuo formula group,high-dose Huayu Jiangzhuo formula group.Using ELISA method to detect hormone levels in rat serum.HE staining was used to detect pathological changes in rat ovarian tissue.Western blot to detect changes in TLR4/NF-κB p56 signaling pathway protein expression levels.Results:After intervention,compared with the model group,body weight and Lee's index of rats in the metformin group,low-dose Huayu Jiangzhuo formula group,high-dose Huayu Jiangzhuo formula group were significantly lower(P＜0.05).Compared with the control group,the levels of testosterone(T),luteinizing hormone(LH),and LH/FSH in the model group rats were significantly higher,while the levels of estradiol(E2),progesterone(P),and follicle stimulating hormone(FSH)were significantly lower(P＜0.05).Compared with the model group,after interven-tion,the T,LH,LH/FSH levels of rats in the metformin group,low-dose Huayu Jiangzhuo formula group and high-dose Huayu Jiangzhuo formula group decreased(P＜0.05),while the E2,P,and FSH levels increased(P＜0.05).Compared with the control group,the relative expression levels of TLR4,MyD88,and NF-κB p65 proteins in the o-varian tissue of the model group rats increased(P＜0.05).Compared with the model group,the relative expression levels of TLR4,MyD88,and NF-κB p65 proteins in the ovarian tissue of rats in the metformin group,low-dose Huayu Jiangzhuo formula group and high-dose Huayu Jiangzhuo formula group were reduced(P＜0.05).Conclusion:Huayu Jiangzhuo formula can regulate secretion level of sex hormones in PCOS rats by inhibiting activity of TLR4/NF-κB p56 signaling pathway and improve ovarian tissue pathological damage.关键词
多囊卵巢综合征/调补脾胃法/痰湿型/炎症/Toll样受体4/核转录因子κBKey words
Polycystic ovary syndrome/Tiaobu Piwei method/Tanshi type/Inflammation/TLR4/NF-κB分类
医药卫生引用本文复制引用
刘博,张成奇,董青,魏萱,王娟,梁贵廷..化瘀降浊方治疗痰湿型多囊卵巢综合征机制研究[J].陕西中医,2024,45(7):888-892,5.基金项目
河北省中医药管理局科研计划项目(2019444) （2019444）
