针刺足三里对小鼠术后肠麻痹的作用机制OACSTPCD

Objective:Study aims to explore effects of acupuncture at Zusanli acupoint on postoperative ileus and its information transmission mechanism.Methods:Ten wild-type mice and ten Toll like receptor 4 gene knockout mice were used to construct postoperative ileus models.The postoperative gastrointestinal motility and intestinal mu-cosal damage were compared.Twenty wild-type mice were used to construct postoperative ileus model,which were randomly divided into electroacupuncture Zusanli acupoint group and control group,with ten mice in each group.The electroacupuncture Zusanli acupoint group were continuously stimulated with electroacupuncture at Zusanli acupoint 4 times at 6 h,24 h,48 h,and 72 h after modeling.The control group were connected to electroacupuncture instru-ment but not given electrical stimulation.The gastrointestinal motility function,levels of inflammatory factors in small intestine tissue,and key molecule Toll like receptor 4 related to Toll like receptor pathways in both groups were detected TRAF6 and JNK expression.Results:Compared with wild-type mice,Toll like receptor 4 gene knockout mice showed improved gastrointestinal motility,down-regulated expression of inflammatory factors in small intestine tissue,and milder intestinal mucosal damage.Stimulating Zusanli acupoint with electroacupuncture could improve gastrointestinal motility,reduce expression levels of inflammatory factors in small intestine tissue,and down-regulate the expression levels of Toll like receptor 4 related factorsTLR4,TRAF6 and JNK.Conclusion:Electroacupuncture at Zusanli acupoint could alleviate postoperative intestinal paralysis,and the Toll like receptor 4 signaling pathway might play key regulatory role in the process.