陕西中医2024，Vol.45Issue(7)：897-901,5.DOI:10.3969/j.issn.1000-7369.2024.07.007
酸枣仁汤调节PI3K/AKT/BDNF信号通路改善围绝经期大鼠失眠作用机制
Action mechanism of Suanzaoren decoction regulates PI3K/AKT/BDNF signaling pathway to improve insomnia in perimenopausal rats
摘要
Abstract
Objective:To explore improvement effect of Suanzaoren decoction on perimenopausal insomnia rats by regulating phosphatidylinositol 3 kinase(PI3K)/protein kinase B(AKT)/brain derived neurotrophic factor(BD-NF)signaling pathway.Methods:Perimenopausal insomnia rat model were established by removing ovaries.The rats were randomly divided into insomnia group,different doses(3.25,7.50,15 g/kg)of Suanzoren decoction group,in-hibitor[2 ng/(ml·kg)]combined with 15 g/kg Suanzoren decoction group,and rats with peri-ovarian adipocytes ex-cised were used as sham operation group.The rats with adipose tissue around ovaries were used as the sham surgery group.General condition of rats were observed,and sleep quality of rats evaluated.ELISA was applied to detect levels of follicle stimulating hormone(FSH)and estradiol(E2)in the serum of each group.Immunohistochemistry was ap-plied to detect expression of serotonin(5-HT)in brain tissue.qRT-PCR and Western blot were applied to detect ex-pression of genes and proteins related to the PI3K/AKT/BDNF pathway.Results:Compared with the sham surgery group,sleep latency,E2,5-HT expression in brain tissue,PI3K mRNA,AKT mRNA,BDNF mRNA expression,and corresponding protein expression were obviously reduced in the insomnia group,while sleep duration and FSH were obviously increased(P＜0.05).Compared with the insomnia group,after intervention with different doses of Suanza-oren decoction,sleep latency,E2,5-HT expression in brain tissue,PI3K mRNA,AKT mRNA,BDNF mRNA expres-sion and corresponding protein expression were obviously increased,while sleep duration and FSH were obviously de-creased,the intervention effect of 15 g/kg Suanzaoren decoction was the best(P＜0.05).Compared with the 15 g/kg Su-anzaoren decoction group,the sleep latency,E2,5-HT expression in brain tissue,PI3K mRNA,AKT mRNA,BDNF mRNA expression and corresponding protein expression were obviously reduced in the combination group,while sleep duration and FSH were obviously increased(P＜0.05).Conclusion:Suanzaoren decoction can effectively im-prove symptoms of insomnia in perimenopausal rats by regulating PI3K/AKT/BDNF signaling pathway.关键词
失眠/酸枣仁汤/脑源性神经生长因子/磷脂酰肌醇3激酶/围绝经期Key words
Insomnia/Suanzaoren decoction/Brain derived neurotrophic factor/Phosphatidyl inositol 3 kinase/Peri-menopause分类
医药卫生引用本文复制引用
邓元香,彭雄,贺婷..酸枣仁汤调节PI3K/AKT/BDNF信号通路改善围绝经期大鼠失眠作用机制[J].陕西中医,2024,45(7):897-901,5.基金项目
湖南省优秀青年科学基金资助项目(2020JJ4469) （2020JJ4469）
