陕西中医2024，Vol.45Issue(8)：1011-1015,1025,6.DOI:10.3969/j.issn.1000-7369.2024.08.001
保肾通络方对糖尿病肾病小鼠肾小管间质纤维化及上皮细胞间充质转分化的影响
Effect of Baoshen Tongluo decoction on renal tubular interstitial fibrosis and epithelial-mesenchymal transition in DN mice
摘要
Abstract
Objective:To observe the effect of Baoshen Tongluo decoction on renal tubular interstitial fibrosis and epithelial-mesenchymal transition in DN mice.Methods:The model KK-Ay mice were separated into DN group,XST group and BSTL group randomly.C57 BL/6J mice performed for NC group.Mice in BSTL group were given BSTL.Mice in XST group were given XST.Mice in DN and NC group were given distilled water.All mice were suc-cessive administration for 12 weeks.Serum creatinine,urea nitrogen and 24 h urine protein was detected.Renal tubu-lar interstitial fibrosis was detected by HE,PAS and Masson staining.Immunohistochemistry and RT-PCR were used for detecting Collagen Ⅰ,FN,α-SMA and E-cadherin protein and mRNA expression.Results:Serum creatinine and urea nitrogen levels of mice in DN group were significantly higher than those in NC group(all P<0.05),while se-rum creatinine and urea nitrogen levels of mice in BSTL group and XST group were significantly lower than those in DN group(all P<0.05).Compared with NC group,the renal tubules in the DN group showed obvious interstitial fi-brosis,epithelial cell exfoliation and vacuolar degeneration,while the above pathological changes were significantly re-duced in BSTL group and XST group.The expression of Collagen Ⅰ and FN protein and mRNA in renal tubules of mice in DN group were significantly up-regulated compared with those in NC group(all P<0.05),while the expres-sion of Collagen Ⅰ and FN protein and mRNA in renal tubules of mice in BSTL group and XST group were signifi-cantly down-regulated compared with those in DN group(all P<0.05).Compared with NC group,the expression of α-SMA protein and mRNA in renal tubular epithelial cells in DN group was significantly up-regulated,and the ex-pression of E-cadherin protein and mRNA was significantly down-regulated(all P<0.05).Compared with DN group,α-SMA protein and mRNA expression in BSTL group and XST group were significantly down-regulated,while E-cadherin protein and mRNA expression were significantly up-regulated(all P<0.05).Conclusion:Baoshen Tongluo decoction can alleviate proteinuria and ameliorate renal function of DN,which partially through decreasing renal tubule interstitial fibrosis and epithelial-mesenchymal transition in DN.关键词
糖尿病肾病/保肾通络方/益肾活血通络/肾小管上皮细胞/间充质转分化/间质纤维化Key words
Diabetic nephropathy/Baoshen Tongluo decoction/Yishen Huoxue Tongluo/Renal tubular epithe-lial cells/Epithelial-mesenchymal transition/Interstitial fibrosis分类
医药卫生引用本文复制引用
崔方强,江心灿,王悦芬,孟元,蔡朕,赵文景..保肾通络方对糖尿病肾病小鼠肾小管间质纤维化及上皮细胞间充质转分化的影响[J].陕西中医,2024,45(8):1011-1015,1025,6.基金项目
国家自然科学基金资助项目(81703989) （81703989）
北京市自然科学基金资助项目(7222271) （7222271）
首都医科大学科研培育基金资助项目(PYZ22163) （PYZ22163）
