陕西中医2024，Vol.45Issue(8)：1016-1020,5.DOI:10.3969/j.issn.1000-7369.2024.08.002
推拿通过NF-κB/GT通路对minor CCI大鼠的镇痛启动机制研究
Explore the analgesic initiation mechanism of tuina in minor CCI rats with NF-κB/GT pathway as the main pathway
摘要
Abstract
Objective:To investigate how tuina helps to start pain relief in treating neuropathic pain.Methods:32 male rats were randomly assigned to four groups:sham group,model group,tuina group,and tuina+PDTC group.A minor CCI model was created in the model group,tuina group,and tuina+PDTC group.Seven days after modeling,the tuina group and tuina+PDTC group received treatment using three methods and three points once.The mechani-cal and thermal pain thresholds of the rats were measured using the Von Frey method,and molecular changes related to the NF-κB/GT pathway in the rat SDH were analyzed through dual immunofluorescence labeling,Western blot,and RT-PCR.Results:After one treatment,the MWT and TWL values,EAAT2 cell number,protein,and mRNA ex-pression were significantly higher in the tuina group and tuina+PDTC group compared to the model group(all P<0.05).Conversely,the GFAP cell number,protein,mRNA expression,and NF-κB mRNA expression were signifi-cantly lower(all P<0.05).Compared with the tuina group,the MWT and TWL values,the number of EAAT2 cells,protein and mRNA expression in the tuina+PDTC group were increased(all P<0.05),GFAP cell number,protein,mRNA expression and NF-κB mRNA expression were decreased(all P<0.05).Conclusion:Tuina can pro-vide immediate pain relief by controlling the NF-κB/GT signaling pathway.关键词
神经病理性疼痛/推拿/镇痛/脊髓背角/中枢机制/NF-κB/GT通路Key words
Neuropathic pain/Tuina/Analgesia/Spinal dorsal horn/Central mechanism/NF-κB/GT pathway分类
医药卫生引用本文复制引用
萨出拉,杨震杰,于天源,陈金平,张润龙,张英琦,孙佳伟,张汉钰,刘家玥..推拿通过NF-κB/GT通路对minor CCI大鼠的镇痛启动机制研究[J].陕西中医,2024,45(8):1016-1020,5.基金项目
国家自然科学基金资助项目(82074573,82274675) （82074573,82274675）
北京市自然科学基金资助项目(7232278) （7232278）
