推拿通过NF-κB/GT通路对minor CCI大鼠的镇痛启动机制研究OACSTPCD

Objective:To investigate how tuina helps to start pain relief in treating neuropathic pain.Methods:32 male rats were randomly assigned to four groups:sham group,model group,tuina group,and tuina+PDTC group.A minor CCI model was created in the model group,tuina group,and tuina+PDTC group.Seven days after modeling,the tuina group and tuina+PDTC group received treatment using three methods and three points once.The mechani-cal and thermal pain thresholds of the rats were measured using the Von Frey method,and molecular changes related to the NF-κB/GT pathway in the rat SDH were analyzed through dual immunofluorescence labeling,Western blot,and RT-PCR.Results:After one treatment,the MWT and TWL values,EAAT2 cell number,protein,and mRNA ex-pression were significantly higher in the tuina group and tuina+PDTC group compared to the model group(all P<0.05).Conversely,the GFAP cell number,protein,mRNA expression,and NF-κB mRNA expression were signifi-cantly lower(all P<0.05).Compared with the tuina group,the MWT and TWL values,the number of EAAT2 cells,protein and mRNA expression in the tuina+PDTC group were increased(all P<0.05),GFAP cell number,protein,mRNA expression and NF-κB mRNA expression were decreased(all P<0.05).Conclusion:Tuina can pro-vide immediate pain relief by controlling the NF-κB/GT signaling pathway.