陕西中医2024，Vol.45Issue(8)：1026-1030,5.DOI:10.3969/j.issn.1000-7369.2024.08.004
慈苓化浊方对痛风大鼠滑膜组织环氧化酶-2、热休克蛋白70表达的影响
Effect of Ciling Huazhuo formula on the expression of COX-2 and HSP70 in synovial tissues of gout rats
摘要
Abstract
Objective:To analyze the effect of Ciling Huazhuo formula on the expression of cyclooxygenase-2(COX-2)and heat shock protein 70(HSP70)in synovium of gout rats.Methods:50 SPF grade SD rats were select-ed,with 10 rats as the control group and an additional 40 rats as the gout model.The 30 successfully modeled rats were divided into a model group,a drug control group,and a Ciling Huazhuo formula group,with 10 rats in each group.The pathological histology,joint swelling,expression of COX-2 and HSP70,inflammatory factors,phosphati-dylinositol 3-kinase(PI3 K)/protein kinase B(AKT)/forkhead box transcription factor O subfamily 1(FoxO1)pathway related factors and their mRNA expression levels were observed in each group.Results:Compared with the control group,the expressions of joint swelling,COX-2,TNF-α,interleukin-1β(IL-1β),interleukin-6(IL-6),PI3 K mRNA,AKT mRNA and FoxO1 mRNA in model group,drug control group and Ciling Huazhuo formula group were increased,the expression levels of HSP70 mRNA and Caspase-3 mRNA were decreased(all P<0.05).Compared with model group,the expressions of joint swelling,COX-2,TNF-α,IL-1β,IL-6,PI3 K mRNA,AKT mRNA and FoxO1 mRNA in drug control group and Ciling Huazhuo formula group decreased,while the expressions of HSP70 mRNA and Caspase-3 mRNA increased(all P<0.05).Compared with drug control group,the expressions of joint swelling,COX-2,TNF-α,IL-1β,IL-6,PI3 K mRNA,AKT mRNA and FoxO1 mRNA in Ciling Huazhuo formula group were decreased,while the expressions of HSP70 mRNA and Caspase-3 mRNA were increased(all P<0.05).Conclusion:The intervention of Ciling Huazhuo decoction in gout rats can reduce the degree of joint swelling,reduce the expression of COX-2 and HSP70 in synovial tissue,and inhibit the inflammatory response.The mechanism of ac-tion may be related to the inhibition of PI3 K/AKT/FoxO1 pathway.关键词
痛风/慈苓化浊方/环氧化酶-2/热休克蛋白70/滑膜/大鼠Key words
Gout/Ciling Huazhuo formula/Cyclooxygenase-2/Heat shock protein 70/Synovium/Rats分类
医药卫生引用本文复制引用
黄愉菊,张剑勇..慈苓化浊方对痛风大鼠滑膜组织环氧化酶-2、热休克蛋白70表达的影响[J].陕西中医,2024,45(8):1026-1030,5.基金项目
国家自然科学基金资助面上项目(82174290) （82174290）
